Ado loss drives TNF-dependent immune evasion.

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Ado loss drives TNF-dependent immune evasion. Ado loss drives TNF-dependent immune evasion. (A and B) Control or Ado knockout MC38 cells were left untreated or treated with TNF (10 ng/ml) for 1 or 6 hours, followed by 3′ RNA-seq. The top regulated genes are displayed with comparisons of control gRNA and Ado knockout (two individual gRNAs for each, performed in duplicate). Representative box plots for the top three TNF-induced genes are displayed. (C) Ado was targeted in MC38Ova with two individual sgRNAs. Prf1+/+ OT-I T cells were then added, and cell death was measured at 18 hours. Data are pooled from three independent experiments. (D) Cells from (C) were treated with TNF (1 ng/ml). At 18 hours, cell death was measured by PI uptake. Data are representative of three independent experiments. (E) Control or Ado-depleted MC38Ova cells were injected subcutaneously into NSG mice. On day 12, and again on day 16, 5 × 106Prf1+/+ OT-I T cells were adoptively transferred. (F) Schematic representation of bystander immune evasion. *P < 0.05, Student’s t test. (G) TCGA data analyses of Ado expression and disease-free survival for different cancer types. Conor J. Kearney et al. Sci. Immunol. 2018;3:eaar3451 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works