Identification of a Commonly Used CDR3 Region of Infiltrating T Cells Expressing Vβ13 and Vβ15 Derived From Psoriasis Patients Ha Young Hwang, Young.

Slides:

Advertisements

Similar presentations

The CXC Receptor 2 Is Overexpressed in Psoriatic Epidermis

Advertisements

by Karen Reue, Robert D. Cohen, and Michael C. Schotz

Quantitative Real-Time Reverse Transcription–Polymerase Chain Reaction Analysis of Drug Metabolizing and Cytoprotective Genes in Psoriasis and Regulation.

Oxidative Activity of the Type 2 Isozyme of 17β-Hydroxysteroid Dehydrogenase (17β- HSD) Predominates in Human Sebaceous Glands Diane Thiboutot, Patricia.

Jonathan A. Schumacher, Stephen D. Jenson, Kojo S. J

Kei Kuroda, Allen Sapadin, Toru Shoji, Raul Fleischmajer, Mark Lebwohl

HAX-1, Identified by Differential Display Reverse Transcription Polymerase Chain Reaction, Is Overexpressed in Lesional Psoriasis Alireza Mirmohammadsadegh,

T Cell Receptor-Vβ Analysis Identifies a Dominant CD60+ CD26– CD49d– T Cell Clone in the Peripheral Blood of Sézary Syndrome Patients Enrico Scala, Dr,

Allergen-specific IgE production of committed B cells from allergic patients in vitro Peter Steinberger, MSca, Barbara Bohlea, Franco di Padova, MDb,

T-Cell Receptor Repertoire and Cytokine Pattern in Granuloma Annulare: Defining a Particular Type of Cutaneous Granulomatous Inflammation Martin Mempel,

Detection of Protein Tyrosine-Kinase (PTK) Gene Expression Pattern in Normal and Malignant T Lymphocytes by Combined PTK-Specific Polymerase Chain Reaction.

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes Marcel B.M. Teunissen, Jan.

UVB Radiation-Mediated Inhibition of Contact Hypersensitivity Reactions Is Dependent on the Platelet-Activating Factor System Qiwei Zhang, Yongxue Yao,

Accumulation of Identical T Cells in Melanoma and Vitiligo-Like Leukoderma Jürgen C. Becker, Per Guldberg, Jesper Zeuthen, Eva-Bettina Bröcker, Per thor.

Expression of Neutrophil Gelatinase-Associated Lipocalin in Skin Epidermis Seong Jun Seo, Ji-Young Ahn, Chang-Kwun Hong, Eun-Young Seo, Kyung-Chae Kye,

Bulge- and Basal Layer-Specific Expression of Fibroblast Growth Factor-13 (FHF-2) in Mouse Skin Mitsuko Kawano, Satoshi Suzuki, Masashi Suzuki, Junko.

Cholinergic Urticaria: Studies on the Muscarinic Cholinergic Receptor M3 in Anhidrotic and Hypohidrotic Skin Yu Sawada, Motonobu Nakamura, Toshinori.

Conversion of the CD4+ T cell profile from TH2-dominant type to TH1-dominant type after varicella-zoster virus infection in atopic dermatitis Takao Fujimura,

Natural Gene Therapy May Occur in All Patients with Generalized Non-Herlitz Junctional Epidermolysis Bullosa with COL17A1 Mutations Anna M.G. Pasmooij,

Th17 Cytokines Stimulate CCL20 Expression in Keratinocytes In Vitro and In Vivo: Implications for Psoriasis Pathogenesis Erin G. Harper, Changsheng Guo,

Identification of cDNA Encoding a Serine Protease Homologous to Human Complement C1r Precursor from Grafted Mouse Skin Sung June Byun, Young Yil Bahk,

The mRNA for Protease Nexin-1 is Expressed in Human Dermal Papilla Cells and its Level is Affected by Androgen Tadashige Sonoda, Yuji Asada, Sotaro Kurata,

Regulation of UVB-Induced IL-8 and MCP-1 Production in Skin Keratinocytes by Increasing Vitamin C Uptake via the Redistribution of SVCT-1 from the Cytosol.

Robyn P. Hickerson, Sancy A. Leachman, Lana N

Expression of Two Breast-Specific Molecules in the Lung

Type II Collagen Accumulation in Overlying Dermo-Epidermal Junction of Pilomatricoma Is Mediated by Bone Morphogenetic Protein 2 and 4 Hideki Mieno,

Interferon α-Inducible Protein 27 (IFI27) is Upregulated in Psoriatic Skin and Certain Epithelial Cancers Sari Suomela, Li Cao, Anne Bowcock, Ulpu Saarialho-Kere,

Katrin Pauls, Margarete Schön, Robert C

Novel Functions of Intracellular IL-1ra in Human Dermal Fibroblasts: Implications in the Pathogenesis of Fibrosis Siva Kanangat, Arnold E. Postlethwaite,

Direct Evidence to Support the Role of Antigen-Specific CD8+ T Cells in Melanoma- Associated Vitiligo Frédérique-Anne Le Gal, Philippe Lefebvre, Jean-Christophe.

The TCR δ Repertoire in Normal Human Skin is Restricted and Distinct from the TCR δ Repertoire in the Peripheral Blood Wolfgang Holtmeier, Michael Pfänder,

Suzanne M. Morgan, Elizabeth Hodges, Tracey J

Identification and Sequencing of a Putative Variant of Proopiomelanocortin in Human Epidermis and Epidermal Cells in Culture Gong Can, Zalfa Abdel-Malek,

Identification of COL7A1 Alternative Splicing Inserting 9 Amino Acid Residues Into the Fibronectin Type III Linker Domain Daisuke Sawamura, Maki Goto,

Inverse Regulation of the Nuclear Factor-κB Binding to the p53 and Interleukin-8 κB Response Elements in Lesional Psoriatic Skin Claus Johansen, Esben.

Isolation and Characterization of a Putative Keratin-Associated Protein Gene Expressed in Embryonic Skin of Mice Mikiro Takaishi, Yoshimi Takata, Toshio.

Pityriasis Rosea is Associated with Systemic Active Infection with Both Human Herpesvirus-7 and Human Herpesvirus-6 Takahiro Watanabe, Tatsuyoshi Kawamura,

Induction of Metallothionein in Human Skin by Routine Exposure to Sunlight: Evidence for a Systemic Response and Enhanced Induction at Certain Body Sites

Μ-Crystallin, Thyroid Hormone-binding Protein, is Expressed Abundantly in the Murine Inner Root Sheath Cells Noriaki Aoki, Kaoru Ito, Masaaki Ito Journal.

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Expression and localization of histamine H2 receptor messenger RNA in human nasal mucosa Noriko Hirata, MDa, Kazuhiko Takeuchi, MDa, Kotaro Ukai, MDa,

Functional Characterization of IL-17F as a Selective Neutrophil Attractant in Psoriasis Hideaki Watanabe, Mio Kawaguchi, Sawa Fujishima, Miyoko Ogura,

Interleukin-13 Receptor in Psoriatic Keratinocytes: Overexpression of the mRNA and Underexpression of the Protein Juan C. Cancino-Díaz, Elba Reyes-Maldonado,

The Paracrine Role of Stem Cell Factor/c-kit Signaling in the Activation of Human Melanocytes in Ultraviolet-B-Induced Pigmentation Akira Hachiya, Akemi.

Caspase-5 Expression Is Upregulated in Lesional Psoriatic Skin

Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27 M. Garmyn, A. Pupe Journal of Investigative Dermatology

RT-PCR for Tyrosinase-mRNA-Positive Cells in Peripheral Blood: Evaluation Strategy and Correlation with Known Prognostic Markers in 123 Melanoma Patients

Gereon Lauer, Stephan Sollberg, Melanie Cole, Thomas Krieg, Sabine A

Bikunin, a Serine Protease Inhibitor, is Present on the Cell Boundary of Epidermis Cui Chang-Yi, Yoshinori Aragane, Akira Maeda, Piao Yu-Lan, Masae Takahashi,

Yoshiharu Kawaguchi Journal of Investigative Dermatology

Connective Tissue Growth Factor: Expression in Human Skin In Vivo and Inhibition by Ultraviolet Irradiation Taihao Quan, Tianyuan He, Sewon Kang, John.

Expression of Interleukin-12 is Increased in Psoriatic Skin

Transcriptional Profiling of Psoriasis Using RNA-seq Reveals Previously Unidentified Differentially Expressed Genes Ali Jabbari, Mayte Suárez-Fariñas,

Human Natural Killer T Cells Infiltrate into the Skin at Elicitation Sites of Allergic Contact Dermatitis Michael D. Gober, Rita Fishelevich, Yuming.

Expression of Opsin Molecule in Cultured Murine Melanocyte

Alterations of Glucosylceramide-β-Glucosidase Levels in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Sabine Pfister, Elisabeth.

The Level of Prosaposin is Decreased in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Silke Stachowitz, Johannes Ring, Heidrun.

Astrid Kehlen Journal of Investigative Dermatology

The Human Dermokine Gene: Description of Novel Isoforms with Different Tissue- Specific Expression and Subcellular Location Eve Toulza, Marie-Florence.

SLURP1 Is a Late Marker of Epidermal Differentiation and Is Absent in Mal de Meleda Bertrand Favre, Laure Plantard, Lorène Aeschbach, Noureddine Brakch,

Identification of Skn-1n, a Splice Variant Induced by High Calcium Concentration and Specifically Expressed in Normal Human Keratinocytes Koji Nakajima,

Lack of Membrane Expression of Interleukin-2 Receptor α Chain (CD25) in Mycosis Fungoides: Application of Laser Scanning Cytometry for Phenotyping of.

David A. Norris, Brian L. Kotzin Journal of Investigative Dermatology

Giorgio Sirugo, Kenneth K. Kidd The American Journal of Human Genetics

Permeability Barrier Disruption Increases the Level of Serine Palmitoyltransferase in Human Epidermis Francesca Alessandrini, Dr., Heidrun Behrendt

The Spectrum of Mitochondrial DNA Deletions is a Ubiquitous Marker of Ultraviolet Radiation Exposure in Human Skin Amanda J. Ray, Richard Turner, Osamu.

Kristiina Airola, Norbert E. Fusenig

Redistribution of LRIG Proteins in Psoriasis

An epigenetic disorder may cause aberrant expression of aromatase gene in endometriotic stromal cells Masao Izawa, Ph.D., Tasuku Harada, M.D., Fuminori.

Transcriptional Activation of Endogenous Retroviral Sequences in Human Epidermal Keratinocytes by UVB Irradiation Christine Hohenadl, Herbert Germaier,

Presentation transcript:

Identification of a Commonly Used CDR3 Region of Infiltrating T Cells Expressing Vβ13 and Vβ15 Derived From Psoriasis Patients Ha Young Hwang, Young Yil Bahk, Tae-Yoon Kim Journal of Investigative Dermatology Volume 120, Issue 3, Pages 359-364 (March 2003) DOI: 10.1046/j.1523-1747.2003.12055.x Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 RT-PCR analysis of expression of TCR β-chain. Total RNA from skin biopsies from six psoriasis patients was reverse transcribed to cDNA. cDNA, which was normalized with GAPDH, was used for the first round PCR with Vβ-specific primer and Cβ3 primer in a total of 23 cycles. The second round PCR with 0.5 μl of the first round PCR product, Vβ-specific primer, and Cβ5 primer (internal primer of constant region) was carried out for 25 cycles. The PCR products were analyzed on a 1.3% agarose gel. N means nonlesional skin and L means lesional skin from psoriasis patients. Arrows in right size indicate the real PCR products. Journal of Investigative Dermatology 2003 120, 359-364DOI: (10.1046/j.1523-1747.2003.12055.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Detection of the CDR3 motif (Vβ13-DWTSGV-Jβ2.7) in skin biopsies derived from seven psoriasis patients (patients 7-13) and six healthy individuals (H1-H8). cDNA was prepared from total RNA from 2mm skin biopsies by reverse transcription and normalized with GAPDH. Normalized cDNA was first amplified using Vβ13 primer and Cβ3 primer. The second round PCR was conducted using oligonucleotide corresponding to DWTSGV-Jβ2.7 and Cβ3 for CDR3, and Cβ2 and Cβ3 primer for the constant region as internal control, respectively. The PCR products were separated on a 1.3% agarose gel and transferred to a positively charged nylon membrane and covalently fixed by ultraviolet light. Then, the membrane was prehybridized, and hybridized with 32P-radiolabeled oligonucleotide probe corresponding to the Vβ13-DWTSGV-Jβ2.7 at 56°C and Cβ5 specific for the constant region at 44°C. After washing, the membrane was analyzed after exposure on X-ray film. Journal of Investigative Dermatology 2003 120, 359-364DOI: (10.1046/j.1523-1747.2003.12055.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Detection of the CDR3 motif Vβ13-DWTSGV-Jβ2.7 in skin biopsy specimens of nonlesional skin (N) and psoriatic lesional skin (L) derived from psoriasis patients. Skin biopsies obtained from 15 psoriasis patients (patients 14-28) were analyzed under the same conditions as described in Fig 2. Journal of Investigative Dermatology 2003 120, 359-364DOI: (10.1046/j.1523-1747.2003.12055.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Detection of the CDR3 motif (Vβ13-DWTSGV-Jβ2.7) in PBMC and in CLA+ cells from psoriasis patients and healthy individuals. PBMC was isolated from heparinized blood by density centrifugation using Ficoll-Hypaque. CLA+ cells were purified from PBMC by the magnetic cell separation method as described in Materials and Methods. The existence of the specific CDR3 motif in PBMC obtained from seven patients with psoriasis (patients 22-28) and three healthy individuals (H1-H3) was analyzed, and in CLA+ cells from five patients with psoriasis (patients 29-33) and three healthy individuals (H4-H6) as previously mentioned. NC means negative control and PC means positive control. (A) Detection of the CDR3 motif in PBMC and (B) detection of the CDR3 motif in CLA+ cells. Journal of Investigative Dermatology 2003 120, 359-364DOI: (10.1046/j.1523-1747.2003.12055.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions