Comparison of the original 515f/806r primer pair and the new, modified 515f/806rB primer pair. Comparison of the original 515f/806r primer pair and the.

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Comparison of the original 515f/806r primer pair and the new, modified 515f/806rB primer pair. Comparison of the original 515f/806r primer pair and the new, modified 515f/806rB primer pair. (A) Procrustes plot of original and modified 515f/806r constructs, with unweighted UniFrac metric, M2 = 0.111. (B) Original and modified 515f/806r constructs, weighted UniFrac metric M2 = 0.196. (C) Pie charts illustrating the mean relative abundance of phyla present (all studies combined) in samples amplified with the old 515f/806r construct or with the modified 515f/806rB construct. (D) Taxa scatterplots for the original and modified 515f/806r primers. Phyla plots are shown for American Gut fecal, American Gut skin, agricultural soils, EMP Rice Rhizome, Body Farm 1, Body Farm 2, mouse decomposition, and Sloan built environment samples. Outlier samples have been removed from the results shown. William Walters et al. mSystems 2016; doi:10.1128/mSystems.00009-15