Sidhu P. Gangadharan, MD, Mohammad H. Eslami, MD, Inanna P

Slides:

Advertisements

Similar presentations

Grafting an Acellular 3-Dimensional Collagen Scaffold Onto a Non-transmural Infarcted Myocardium Induces Neo-angiogenesis and Reduces Cardiac Remodeling

Advertisements

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin Todd K. Rosengart, MD, Kurt.

Mohammad H. Eslami, MDa, Sidhu P

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

High mobility group box 1 promotes endothelial cell angiogenic behavior in vitro and improves muscle perfusion in vivo in response to ischemic injury

Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,

Lysyl oxidase expression in a rat model of arterial balloon injury

Randolph L. Geary, MD, Seppo T. Nikkari, MD, William D. Wagner, PhD, J

Michael A. Rosenbaum, MD, Pinaki Chaudhuri, PhD, Linda M. Graham, MD

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold

Interleukin 18 binding protein (IL18-BP) inhibits neointimal hyperplasia after balloon injury in an atherosclerotic rabbit model Jian-Ming Li, MD, Mohammad.

The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro Carol J. Sawmiller, MD, Richard J. Powell, MD,

Foxc2 overexpression enhances benefit of endothelial progenitor cells for inhibiting neointimal formation by promoting CXCR4-dependent homing Dujuan.

Ryan M. McEnaney, MD, Ankur Shukla, MD, Michael C

Karen M. Newman, PhD, Jessie Jean-Claude, MD, Hong Li, MS, John V

Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M

Adenovirus-mediated intra-arterial delivery of cellular repressor of E1A-stimulated genes inhibits neointima formation in rabbits after balloon injury

The role of circulating cells in the healing of vascular prostheses

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Origin of endothelial cells that line expanded polytetrafluoroethylene vascular grafts sodded with cells from microvascularized fat Stuart K. Williams,

Ian S. Zagon, PhDa, Frank M. Essis, MSa, Michael F

Sandro Lepidi, MD, Richard D. Kenagy, PhD, Elaine W

Percutaneous transplantation of genetically-modified autologous fibroblasts in the rabbit femoral artery: a novel approach for cardiovascular gene therapy

Recombinant human thrombomodulin inhibits arterial neointimal hyperplasia after balloon injury Jian-ming Li, MD, Michael J Singh, MD, Mazen Itani, MD,

Chronic in vitro shear stress stimulates endothelial cell retention on prosthetic vascular grafts and reduces subsequent in vivo neointimal thickness

CD34 affinity pheresis attenuates a surge among circulating progenitor cells following vascular injury Adriana Harbuzariu, MD, Justine Kim, BS, E. Michael.

Fogarty and percutaneous transluminal coronary angioplasty balloon injury induce comparable damage to the arterial wall but lead to different healing.

Nitric oxide–releasing biopolymers inhibit thrombus formation in a sheep model of arteriovenous bridge grafts Paul S. Fleser, MD, Vijay K. Nuthakki,

Improved retroviral transduction efficiency of vascular cells in vitro and in vivo during clinically relevant incubation periods using centrifugation.

Michael A. Rosenbaum, MD, Keiko Miyazaki, MD, Scott M. Colles, Linda M

Midkine is expressed by infiltrating macrophages in in-stent restenosis in hypercholesterolemic rabbits Hiroshi Narita, MD, Sen Chen, PhD, Kimihiro Komori,

Reendothelialization of isolated segments of the canine carotid artery with reference to the possible role of the adventitial vasa vasorum Qun Shi, MD,

Nick D. Tsihlis, PhD, Jozef Murar, BA, Muneera R. Kapadia, MD, Sadaf S

Antisense basic fibroblast growth factor gene transfer reduces early intimal thickening in a rabbit femoral artery balloon injury model David G. Neschis,

Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype Hector F. Simosa, MD, Grace Wang, MD, XinXin Sui,

Gene delivery to in situ veins: Differential effects of adenovirus and adeno-associated viral vectors Mohammad H. Eslami, MD, Sidhu P. Gangadharan, MD,

Differences in reendothelialization after balloon catheter removal of endothelial cells, superficial endarterectomy, and deep endarterectomy Steven G.

Volume 58, Issue 3, Pages (September 2000)

Local treatment with recombinant tissue factor pathway inhibitor reduces the development of intimal hyperplasia in experimental vein grafts Tam T.T.

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin Todd K. Rosengart, MD, Kurt.

The temporal relationship between the development of vein graft intimal hyperplasia and growth factor gene expression John R. Hoch, MD, Vida K. Stark,

Delayed inhaled carbon monoxide mediates the regression of established neointimal lesions Michael Madigan, MD, Fateh Entabi, MD, Brian Zuckerbraun, MD,

A chemically modified dextran inhibits smooth muscle cell growth in vitro and intimal in stent hyperplasia in vivo Jean-François Deux, MDa,b, Sandrine.

Marcel Scheinman, MD, Enrico Ascher, MD, Gabriel S

In vitro construction of a human blood vessel from cultured vascular cells: A morphologic study Nicolas L'Heureux, MSc, Lucie Germain, PhD, Raymond Labbé,

Retroviral-mediated transduction of endothelial cells with the lac Z gene impairs cellular proliferation in vitro and graft endothelialization in vivo

Aortic wall cell proliferation via basic fibroblast growth factor gene transfer limits progression of experimental abdominal aortic aneurysm Katsuyuki.

Sidhu P. Gangadharan, BS, Robert J. Wagner, BS, Jack L. Cronenwett, MD

Thrombolysis for experimental deep venous thrombosis maintains valvular competence and vasoreactivity Jeffrey M. Rhodes, MD, Jae-Sung Cho, MD, Peter.

Michael A. Rosenbaum, MD, Keiko Miyazaki, MD, Linda M. Graham, MD

John Blebea, MD, Robert A. Cambria, MD, David DeFouw, PhD, Richard N

Transient exposure to elastase induces mouse aortic wall smooth muscle cell production of MCP-1 and RANTES during development of experimental aortic aneurysm

The effects of low-dose radiation on neointimal hyperplasia

Michael Boyer, MD, Laurace E. Townsend, PhD, L

Yehuda G. Wolf, MD, Lars M. Rasmussen, MD, Yoav Sherman, MD, Warner P

Transarterial wall oxygen gradients at a prosthetic vascular graft to artery anastomosis in the rabbit Steven M. Santilli, MD, PhD, Shane E. Wernsing,

Boulos Toursarkissian, MD, David Schwartz, MD, PhD, Paul R

The natural history of microsurgical lymphovenous anastomoses: An experimental study Peter Gloviczki, M.D., Larry H. Hollier, M.D., Frederick E. Nora,

Endothelial cell harvest for seeding vascular prostheses: The influence of technique on cell function, viability, and number Bernard M. Bourke, M.B.B.S.,

Preseeding with autologous fibroblasts improves endothelialization of glutaraldehyde- fixed porcine aortic valves Helmut Gulbins, MD, Angelika Goldemund,

Alexander S. Tretinyak, MD, Eugene S. Lee, MD, Kristina M

Changes in the cooling rate and medium improve the vascular function in cryopreserved porcine femoral arteries Montserrat Rigol, PhD, Magda Heras, MD,

Nick D. Tsihlis, PhD, Jozef Murar, BA, Muneera R. Kapadia, MD, Sadaf S

Joseph J. Piotrowski, MD, Glenn C. Hunter, MD, Cleamond D

Derek T. Woodrum, BS, Karl F. Welke, MD, Mark F. Fillinger, MD

Adenoviral-mediated uteroglobin gene transfer inhibits neointimal hyperplasia after balloon injury in the rat carotid artery Robert A. Larson, MD, Mina.

Endoluminal smooth muscle cell seeding limits intimal hyperplasia

Prevention of stenosis after vascular reconstruction: Pharmacologic control of intimal hyperplasia—A review Alexander W. Clowes, MD, Michael A. Reidy,

Presentation transcript:

Monocyte adhesion to balloon-injured arteries: The influence of endothelial cell seeding Sidhu P. Gangadharan, MD, Mohammad H. Eslami, MD, Inanna P. Weiss, BS, XinXin Sui, MD, Michael S. Conte, MD Journal of Vascular Surgery Volume 33, Issue 6, Pages 1247-1254 (June 2001) DOI: 10.1067/mva.2001.114211 Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 1 Representative analysis of cultured venous ECs for presence of scavenger receptor for LDL. ECs were incubated in vitro with fluorescent di-I-Ac-LDL. A, Appearance with phase contrast optics. (Original magnification × 400.) B, Appearance of cells with rhodamine filter. (Original magnification × 400.) Journal of Vascular Surgery 2001 33, 1247-1254DOI: (10.1067/mva.2001.114211) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 2 Immunohistochemical staining for presence of endothelium, with anti-CD31 monoclonal antibody used as primary antibody. Representative cross sections are shown. A, Mock-seeded artery after balloon denudation. (Original magnification × 1000.) B, EC-seeded artery after balloon injury. (Original magnification × 1000.) Journal of Vascular Surgery 2001 33, 1247-1254DOI: (10.1067/mva.2001.114211) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 3 Confocal microscopy of luminal surface of EC-seeded vessels. ECs were labeled with green fluorescent tracer immediately before seeding to balloon-injured vessels. At sacrifice, vessels were opened longitudinally and examined en face. A, EC-seeded vessel 4 hours after seeding. (Original magnification × 400.) B, Seeded vessel 2 days after seeding. (Original magnification × 400.) Journal of Vascular Surgery 2001 33, 1247-1254DOI: (10.1067/mva.2001.114211) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 4 Appearance of Evans blue–stained, balloon-injured arteries. EC seeding was performed as described in text. Evans blue dye was administered approximately 1 hour before sacrifice at postseeding day 5 to 7. A and B show paired specimens harvested from different animals: top mock; bottom , EC-seeded. (Original magnification × 7.5.) Journal of Vascular Surgery 2001 33, 1247-1254DOI: (10.1067/mva.2001.114211) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 5 51Cr-labeled THP-1 cell adhesion to balloon-injured vessels ex vivo. Paired experiments were performed; ECs were seeded to one balloon-injured iliofemoral segment, contralateral side received medium. Radiolabeled THP-1 cells were introduced into balloon-injured vessels at postinjury day 6 to 9. Average numbers of bound cells/cm2 ± SEM are displayed. Data shown represent 12 individual animal experiments. P < .05. Journal of Vascular Surgery 2001 33, 1247-1254DOI: (10.1067/mva.2001.114211) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 6 En face fluorescence microscopy of rabbit iliac arteries after explanted 1 week after injury and after ex vivo exposure to fluorescent green–labeled THP-1 cells. A, Uninjured artery. B, Balloon-injured vessel, no treatment. C and D, Paired arteries from single animal: EC-seeded artery shown in C ; contralateral mock-seeded vessel shown in D . (Original magnification × 200.) Journal of Vascular Surgery 2001 33, 1247-1254DOI: (10.1067/mva.2001.114211) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 7 RAM-11 staining of balloon-injured vessel, day 7. A, RAM-11 primary antibody. Arrows point to infiltrating macrophages. B, Same vessel with PBS control. (Original magnification × 200.) Journal of Vascular Surgery 2001 33, 1247-1254DOI: (10.1067/mva.2001.114211) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions