Effect of the ERM peptide on actin organization.

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Effect of the ERM peptide on actin organization. Effect of the ERM peptide on actin organization. (A) Cells were either left untreated (CTR) or stimulated with forskolin (FK) with or without preincubation for 3 hours with the ERM peptide or with the control peptide. F-actin was stained with TRITC-conjugated phalloidin and visualized by epifluorescence microscopy. Bar, 5 μm. (B) F-actin quantization by actin polymerization assay. Confluent cells were either left untreated (CTR) or stimulated with forskolin (FK) or preincubated for 3 hours with the ERM peptide. As internal control, cells were incubated with a control peptide with a reversed sequence with respect to the ERM peptide. After staining with TRITC-phalloidin, cells were extracted with cold methanol and the fluorescence absorbance of extracts was read (540/565 nm) in a RF-5301PC fluorimeter. The values obtained were compared by a one-way Anova and Tukey's multiple comparison test (#P<0.001). Grazia Tamma et al. J Cell Sci 2005;118:3623-3630 © The Company of Biologists Limited 2005