SH3 domain of spectrin accumulates in the early integrin clusters.

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Volume 23, Issue 2, Pages (August 2005)

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SH3 domain of spectrin accumulates in the early integrin clusters. SH3 domain of spectrin accumulates in the early integrin clusters. BAE cells were transiently transfected with plasmid encoding HA epitope-tagged SH3 domain. Cells were detached and replated on fibronectin for 1 hour, fixed and permeabilized. Cells were stained with monoclonal antibody against HA epitope and polyclonal antibodies against an autolytic fragment of calpain. (B) Confocal analysis was performed on horizontal (x-y plane) optical sections (0.3 μm intervals); three sections going from the upper to the lower region (left to right) of a single cell are shown. Arrows indicate calpain-fragment- and SH3-domain-containing clusters. Bar, 20 μm (A); 5 μm (B). Katarzyna Bialkowska et al. J Cell Sci 2005;118:381-395 © The Company of Biologists Limited 2005