LacZ activation upon conditional Cx43 deletion.

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LacZ activation upon conditional Cx43 deletion. LacZ activation upon conditional Cx43 deletion. (A) A silent lacZ reporter gene (NLS-lacZ) was integrated into the floxed Cx43 allele. After Cre/loxP-mediated rearrangement of the Cx43-coding region, indicated by loxP sites (triangles), the lacZ gene is activated and the β-galactosidase protein is expressed. (B) The CreERT2 fusion protein is specifically transcribed under control of the SM22α promoter in smooth muscle cells. In the cytosol, CreERT2 is associated with the heat shock protein 90 (HSP 90). After application of the ligand (tamoxifen) this complex dissociates and CreERT2 is translocated into the nucleus, where deletion of the floxed coding region occurs. Britta Döring et al. J Cell Sci 2006;119:1715-1722 © The Company of Biologists Limited 2006