Analysis of centromere and kinetochore function after depletion of DmSMC4. Analysis of centromere and kinetochore function after depletion of DmSMC4. (A)

Slides:

Advertisements

Similar presentations

Section B1: The Mitotic Cell Cycle

Advertisements

(a) Prophase: (1) Chromosomes condense and become visible;

The Cell Cycle & Cell Division

CHAPTER 5 THE CELL CYCLE The Mitotic Cell Cycle 1.The mitotic phase alternates with interphase in the cell cycle: an overview 2. The mitotic spindle distributes.

Objective 4: TSWBAT describe the phases of mitosis including the events characteristic of each phase.

Figure 2.19 Cell Division: Mitosis and Cytokinesis Process Diagrams Step-by-Step Copyright © 2007 by John Wiley & Sons, Inc.

Mitosis Flashcards.

M-Phase Prophase – Metaphase – Anaphase – Telophase Prophase

PROPHASE METAPHASE ANAPHASE TELOPHASE.  Chromosomes condense and nucleoli disappear  Each duplicated chromosome becomes visible as distinct sister chromatids.

The Cell Cycle & Mitosis “Omnis cellula e cellula.” “Every cell from a cell.” —Rudolph Virchow, Germany, 1855.

The Cell Cycle 4 phases exist in the cell cycle for somatic (body) cells. – During cell reproduction the chromatin will condense into chromosomes. We have.

Today’s learning outcome: 1) To learn the different phases of mitosis 2) To be able to identify the stages from images.

Cellular Division in Eukaryotic Cells

Mitosis is the division of the nucleus into two Cytokinesis is the division of the cell into two.

Chromosomes Diploid: contains 2 sets of chromosomes  Body cells, 2n Haploid: contains 1 set of chromosomes  Gametes, sex cells, n Sister chromatids-

Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings The Cell Cycle.

Mitosis A Sequencing Activity.

Danielle Greenwald Emily Lynch Period 4. Mitosis is the process of cell division which results in the production of two daughter cells from a single cell,

Stages of the Cell Cycle

Ch. 5 Part 2 Mitosis. Mitosis  Division of the nucleus that results in two genetically identical daughter cells with the EXACT same number of chromosomes.

8.6 Cell division is a continuum of dynamic changes

The Cell Cycle. What is the cell cycle? The Stages of the life of the cell Involve: 1. Metabolic activities 2. Division.

PROPHASE TELPHASE METAPHASEANAPHASE OVERVIEW Links Glossary Part 2 Glossary Part 1.

Chapter 9 Mitosis and Meiosis Review graphics. Fig µmChromosomes Chromosome duplication (including DNA synthesis) Chromo- some arm Centromere.

Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings.

Cell Division J. Carmona Biology. Interphase  The cell is engaged in metabolic activity and performing its prepare for mitosis (the next four phases.

The Stages of Cell Division BY: MARK DESILVA. Prophase/ Pro Metaphase Anaphase Telophase The Stages of Mitosis.

AP Biology The Cell Cycle Part 2. Centrioles Interphase.

Cell Division: Key Roles

Youngeun Park Helia Hosseinpour

Chromosomes Diploid: contains 2 sets of chromosomes Body cells, 2n

Mitosis Process by which the nucleus of the cell is divided into two nuclei; each with the same number and kinds of chromosomes as the parent cell.

Mitosis is conventionally divided into five phases:

12.2: Mitosis and Interphase of Animals

Chapter 12 – The Cell Cycle

Chapter 12 The Cell Cycle.

CHAPTER 12 – THE CELL CYCLE

Cell Cycle: Mitosis.

Chapter 9 Mitosis.

The Mitotic Spindle: A Closer Look

Megakaryocytes express functional Aurora-B kinase in endomitosis

AP Biology The Cell Cycle Part 2.

Mitosis & Meiosis Mr. Davis.

Cell Division: Mitosis.

Maki Murata-Hori, Yu-li Wang Current Biology

AP Biology The Cell Cycle Part 2.

Mitosis By: Mindy & Fiona.

Volume 11, Issue 5, Pages (March 2001)

Cell Biology I. Overview

Geoffrey J. Guimaraes, Yimin Dong, Bruce F. McEwen, Jennifer G. DeLuca

Volume 113, Issue 7, Pages (June 2003)

Volume 19, Issue 21, Pages (November 2009)

Kinetochore Dynein Is Required for Chromosome Motion and Congression Independent of the Spindle Checkpoint Zhenye Yang, U. Serdar Tulu, Patricia Wadsworth,

10-2 The Cell Cycle.

Volume 13, Issue 3, Pages (February 2003)

Fig. 4. Acentriolar mitotic spindle assembly

Fig. 5. Recovery steps of mitotic acentriolar cells after cold MT depolymerisation.(A,B) Time-lapse sequences of mitotic spindle re-formation at 18°C.

Fig. 3. The checkpoint proteins Mad2 and BubR1 remain associated with the kinetochores of unaligned chromosomes in cenp-metaΔ mutant cells entering anaphase.(A–C)

RECQL4 is a MAP with a spindle function.

The spindle assembly checkpoint acts as a molecular safeguard in ensuring faithful chromosome transmission during mitosis. The spindle assembly checkpoint.

Exo70 is recruited to the plasma membrane at sites of mechanical wounding. Exo70 is recruited to the plasma membrane at sites of mechanical wounding. NRK.

Single MTs can be resolved in subsections of SBF-SEM image stacks.

IGF1R is present in endometrial luminal epithelium at the receptive phase. IGF1R is present in endometrial luminal epithelium at the receptive phase. Endometrial.

CENP-F staining in prometaphase and metaphase spermatocytes from B6 and Rb/+ mice. CENP-F staining in prometaphase and metaphase spermatocytes from B6.

Immunolocalisation of DmSMC4 and Barren in control and DmSMC4-depleted Drosophila S2 cells. Immunolocalisation of DmSMC4 and Barren in control and DmSMC4-depleted.

Tension develops across sister kinetochores (red) upon their bipolar attachment by spindle microtubules (black line). Tension develops across sister kinetochores.

Maytansinol disrupts the mitotic spindle and prevents mitotic exit in Drosophila. Maytansinol disrupts the mitotic spindle and prevents mitotic exit inDrosophila.

Presentation transcript:

Analysis of centromere and kinetochore function after depletion of DmSMC4. Analysis of centromere and kinetochore function after depletion of DmSMC4. (A) Immunofluorescence analysis of CID in control cells shows discrete localisation to centromeres in prometaphase and anaphase. (B) Localisation of CID in DmSMC4-depleted cells shows centromere staining throughout mitosis. In early anaphase cells, CID staining appears to extend polewards beyond the mitotic chromatin (arrows) and in late anaphase centromeres lead migrating chromatids to the spindle poles. After incubation with colchicine, CID-stained centromeres localise over the chromosomes. (C) Control cell immunostained with anti-POLO antibodies show localisation to kinetochores and to centrosomes located at either side of the metaphase plate. (D) After depletion of DmSMC4, POLO localises to kinetochores that during anaphase can also be seen to stretch towards the poles. (E) Control cells stained for CID and tubulin show centromeres associated with microtubule bundles. A higher magnification image is shown in the left panel. (F) In DmSMC4-depleted cells centromeres stretched beyond the chromatin associated with microtubule bundles. Higher magnification images are shown in the left panels. Scale bars: 5 μm. Paula A. Coelho et al. J Cell Sci 2003;116:4763-4776 © The Company of Biologists Limited 2003