Grethe Bergseth, BS, John D

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Artificial Surface-Induced Inflammation Relies on Complement Factor 5: Proof From a Deficient Person Grethe Bergseth, BS, John D. Lambris, PhD, Tom Eirik Mollnes, MD, PhD, Knut Tore Lappegård, MD, PhD The Annals of Thoracic Surgery Volume 91, Issue 2, Pages 527-533 (February 2011) DOI: 10.1016/j.athoracsur.2010.10.084 Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Complement activation and neutrophil degranulation in C5-deficient blood (C5D), C5-deficient blood reconstituted with C5 (C5D-R) and healthy control blood (Control) exposed to polyvinyl chloride. Blood was circulated for 4 hours in closed loops followed by quantification of the (A) terminal complement complex (TCC [AU/mL]), (B) myeloperoxidase (MPO [ng/mL]), (C) lactoferrin (ng/mL), and (D) elastase (ng/mL). Data are presented as mean and range of two separate experiments on two consecutive days. (T0 = baseline values; PBS = phosphate-buffered saline; Comp = compstatin [25 μM]; C5aRA = C5a receptor antagonist [10 μM]); Ctr.p. = control peptide [25 μM].) The Annals of Thoracic Surgery 2011 91, 527-533DOI: (10.1016/j.athoracsur.2010.10.084) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Release of cytokines: (A) release of IL-8 (interleukin 8), (B) MCP-1 (monocyte chemoattractant protein 1), (C) PDGF-BB (platelet-derived growth factor-BB), and (D) VEGF (vascular endothelial growth factor) in C5-deficient blood (C5D), C5-deficient blood reconstituted with C5 (C5D-R), and healthy control blood (Control) exposed to polyvinyl chloride. Blood was circulated for 4 hours in closed loops. Data are presented as mean and range of two separate experiments on two consecutive days. (T0 = baseline values; PBS = phosphate-buffered saline; Comp = compstatin [25 μM]; C5aRA = C5a receptor antagonist [10 μM]); Ctr.p. = control peptide [25 μM].) The Annals of Thoracic Surgery 2011 91, 527-533DOI: (10.1016/j.athoracsur.2010.10.084) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Leukocyte expression of CD11b was determined by median fluorescent intensity (MFI) in flow cytometry on (A) granulocytes and (B) monocytes in C5-deficient blood (C5D), C5-deficient blood reconstituted with C5 (C5D-R), and healthy control blood (Control) exposed to polyvinyl chloride. Blood was circulated for 15 minutes in closed loops. Data are presented as mean and range of two separate experiments on two consecutive days. (T0 = baseline values; PBS = phosphate-buffered saline; Comp = compstatin [25 μM]; C5aRA = C5a receptor antagonist [10 μM]); Ctr.p. = control peptide [25 μM].) The Annals of Thoracic Surgery 2011 91, 527-533DOI: (10.1016/j.athoracsur.2010.10.084) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions