Disruption of the tubule formation by immortalized cells during nephrogenesis in vitro. Disruption of the tubule formation by immortalized cells during.

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Disruption of the tubule formation by immortalized cells during nephrogenesis in vitro. Disruption of the tubule formation by immortalized cells during nephrogenesis in vitro. After dissociation and reaggregation of embryonic kidney, MM cells were mixed at a ratio of 50:1 with either Renca-YFP, HeLa-YFP or mK4-YFP cells. (A-D) The Pax2+ tubular epithelial structures (in red) and glomeruli (arrowheads) were formed from dissociated and reaggregated embryonic kidney mesenchymal progenitor cells. (E-H) Mixing of Renca-YFP cells to MM strongly impaired tubular-structure formation, whereas addition of HeLa-YFP (I-L) or mK4-YFP (M-P) cells had a much milder effect on the nephrogenesis in vitro. 3D cultures were maintained for 4 days. Blue, nuclear stain (Hoechst); green, YFP; red, Pax2 immunostaining. Arrows indicate tubular epithelial structures. Scale bars: 20 µm. Qi Xu et al. Dis. Model. Mech. 2017;10:1503-1515 © 2017. Published by The Company of Biologists Ltd