AP Lab 7 Wisconsin Fast Plants “Who’s The Father?

What are Wisconsin Fast Plants? Read the lab and do p.2 Type of mustard, radish, cabbage plant especially bred by Paul Williams of the University of Wisconsin

WFP Life Cycle Video Fast Plants Time Lapse Video

See stems and ANL (purple) and anl (non-purple or green)

Grow seeds in Petri dish Use Lid as bottom Other part as cover Cut paper towel circle Wet paper towel well with a pipette NAME

Water is to break the seed coat Germinates in a day or two

FRIDAY -Mark Petri dish lid into two halves -Label P on one side, F1 the other -Moisten paper towel circle and place 5 seeds in each side of the lid F2 WFP (1:57) Put you initials on the Petri dish cover P F1

Root emerges with root hairs to increase surface area for water absorption.

Petri dish at slight angle under a light It will sit all week-end so be sure there is enough water in the Petri dish.

Day4 (MONDAY) (cont. on next slide) Stem and leaves emerge Water F1 and P well. “Observing the Seeds” (make 3 quantitative and 3 qualitative observations, sketch and color, count the genotypes and phenotypes.) Attach a paper with these observations.

Monday: Now plant F2 Place 10 seeds in a Petri dish Two rows of 5 Water well. Label F2 Cover Label your initials Put under light at a slight angle 0 0 0 0

Check on seeds –add water if needed Tuesday Take data for F1 and P Check on seeds –add water if needed

Day 7 (Friday) DATA COLLECTION DAY OF F2 Purple/Standard Non-Purple/Standard Purple/Rosette-Dwarf Non-Purple/ Rosette Dwarf (see pictures next slide)

Friday: Count F2 Purple/standard stem Non-purple/Standard stem Rosette dwarf

Collect Group Data P. 6 Sketch plants, color Do dihybrid of the plant genotypes

Chi square Test Bozeman Biology Chi Square Test To test your null hypothesis Critical value and degrees of freedom

Day14-20 (flowers)

How do you grow WFP? Need constant light

Seeds are tiny-do not cover with very much potting soil How do you grow WFP? Seeds are tiny-do not cover with very much potting soil

How do you grow WFP? Need fertilizer

Label your quads

Green and Non-green leaves

Wisconsin Fast Plants® F1 Non-Purple Stem, Yellow-Green Leaf (F1 Anthocyaninless, Yellow-Green)

F1 seed from cross of Non-Purple Stem, Yellow-Green Leaf (anl/ygr) × Purple Stem (ANL/YGR);