Subnuclear localization of HA-tagged EBNA1 protein.

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Subnuclear localization of HA-tagged EBNA1 protein. Subnuclear localization of HA-tagged EBNA1 protein. (A) Cells were processed for immunofluorescence analyses with anti-HA antibody. Images of interphase nuclei counterstained with DAPI (left panel) and anti-HA antibody staining (middle panel) are shown. The merged image is also shown (right panel). (B) Punctate signals of EBNA1 overlap the signals of EBV genomes in the recombinant EBV-infected cells. A combined method of FISH and immunofluorescence was used to visualize EBV genomes (FISH) and HA-tagged EBNA1 simultaneously. Merged images of FISH and immunofluorescence signals are also shown. The images of wild-type EBV-infected cells subjected to the same type of analysis are also shown as controls (bottom). Bars, 5 μm. Teru Kanda et al. J Cell Sci 2007;120:1529-1539 © The Company of Biologists Limited 2007