CD mediates metabolism and efflux of crystal-derived cholesterol

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Online table I: Sequences of primers used. ControlIron RM M2 I ControlIron RM M2 ML Iron *** Iron content (nmol/mg protein) **

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Fig. 4. CD mediates metabolism and efflux of crystal-derived cholesterol. CD mediates metabolism and efflux of crystal-derived cholesterol. (A) Macrophages loaded with CCs prepared from D6-cholesterol (D6-CC) can reduce the amount of free, crystal-derived D6-cholesterol by three main mechanisms. First, acetyl-CoA acetyltransferase (ACAT-1) can catalyze the formation of D6-cholesteryl esters, the storage form of cholesterol, which are deposited in lipid droplets. Second, the mitochondrial enzyme 27-hydroxylase (Cyp27A1) can catalyze the formation of D5-27-hydroxycholesterol, which can passively diffuse across cell membranes. Third, D5-27-hydroxycholesterol is a potent activator of LXR transcription factors, which in turn mediate the up-regulation of the cholesterol efflux transporters ABCA1 and ABCG1. (B and C) iMacs loaded with 200 μg of D6-CC per 1 × 106 cells for 3 hours were treated with 10 mM CD or vehicle control before GC-MS-SIM analysis of crystal-derived cholesterol. (B) Percentage of esterified D6-cholesterol in cell and supernatant fractions before CD treatment (control bar) and after 48 hours of CD treatment. (C) Efflux of D6-cholesterol into supernatants of D6-CC–loaded macrophages before CD treatment (control bar) and upon 24 hours of CD treatment. (D to F) Gene expression of Abca1 and Abcg1 and protein expression of ABCA1 in BMDMs loaded with 100 μg of CC per 1 × 106 cells for 3 hours and then incubated with 10 mM CD or medium control for (D and E) 4 or (F) 24 hours. Immunoblot in (F) is representative of three independent experiments, and densitometric analysis of all three experiments is provided for 10 mM CD and presented as ABCA1 expression relative to the loading control β-actin. Data are shown as means + SEM of at least three independent experiments. (G) D5-27-hydroxycholesterol in cell and supernatant fractions of iMacs loaded with 200 μg of D6-CC per 1 × 106 cells for 3 hours before 48 hours of treatment with 10 mM CD or medium control, determined by GC-MS-SIM. (H) 27-Hydroxycholesterol in cell and supernatant fractions of iMacs after 48 hours of treatment with 10 mM CD or medium control. ***P < 0.001 and *P < 0.05, medium versus CD (B to C); CC + control versus CC + CD (D to F); control versus CD (G and H) (unpaired two-tailed Student’s t test). Sebastian Zimmer et al., Sci Transl Med 2016;8:333ra50 Copyright © 2016, American Association for the Advancement of Science