Morphologic changes induced in immortalized human podocytes after treatment with TGF-β1 (10 ng/mL, right) for 3 days when compared with control cells (left),

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Morphologic changes induced in immortalized human podocytes after treatment with TGF-β1 (10 ng/mL, right) for 3 days when compared with control cells (left), as shown by scanning electron microscopy (A and B, magnification 100×, insert showing microvilli), light microscopy (C and D, magnification 100×), immunofluorescence staining for β-actin (green) and nestin (red) (E and F), and immunofluorescence staining for F-actin (red) and ZO-1 (green) with a blue nuclear counterstain (DAPI) (G and H). Morphologic changes induced in immortalized human podocytes after treatment with TGF-β1 (10 ng/mL, right) for 3 days when compared with control cells (left), as shown by scanning electron microscopy (A and B, magnification 100×, insert showing microvilli), light microscopy (C and D, magnification 100×), immunofluorescence staining for β-actin (green) and nestin (red) (E and F), and immunofluorescence staining for F-actin (red) and ZO-1 (green) with a blue nuclear counterstain (DAPI) (G and H). (A high-quality color representation of this figure is available in the online issue.)‏ Michal Herman-Edelstein et al. Diabetes 2011;60:1779-1788 ©2011 by American Diabetes Association