Role of Src and PTP-PEST in upregulation of tyrosine phosphorylation of paxillin and FAK. (A) Mock and D11 cells were pre-incubated with PP2 or PP3 (10 µM,

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Role of Src and PTP-PEST in upregulation of tyrosine phosphorylation of paxillin and FAK. (A) Mock and D11 cells were pre-incubated with PP2 or PP3 (10 µM, 2 hours), and subjected to immunoblotting with the indicated anti-paxillin antibodies. Role of Src and PTP-PEST in upregulation of tyrosine phosphorylation of paxillin and FAK. (A) Mock and D11 cells were pre-incubated with PP2 or PP3 (10 µM, 2 hours), and subjected to immunoblotting with the indicated anti-paxillin antibodies. (B) Cells were transfected with PTP-PEST or empty (Ctr) vectors, and transfectants were analysed by immunoblotting with anti-PTP-PEST, anti-paxillin or anti-FAK antibodies. (C) Control or PTP-PEST transfectants were analysed by immunoblotting for Erk1/2 and pErk1/2 expression. (D) Transfectants were analysed by confocal microscopy for paxillin expression (left). Also shown is the quantification of FA phenotypes from the confocal images (right). (E) Control or PTP-PEST transfectants were subjected to invasion across Matrigel in response to CXCL12. (**P<0.01). Scale bars: 25 µm. Georgina P. Coló et al. J Cell Sci 2012;125:5338-5352 © 2012. Published by The Company of Biologists Ltd