Effects of the Pax4 mutation on Pax6 transcription activity in COS7 cells. Effects of the Pax4 mutation on Pax6 transcription activity in COS7 cells. The amount of reporter plasmids (5 × P6CON-TK-Luc) was 100 ng (lanes 1–4). The pcDNAPax6 was 50 ng (lane 2). The pcDNAPax4(Wild) (or R121W) was also 50 ng (lanes 3 and 4). The nonrecombinant plasmid (pcDNA 3.1) was cotransfected to adjust the total amount of DNA (200 ng) (lanes 1 and 2; 100 and 50 ng, respectively). *P < 0.01, n = 8. Yoshinori Shimajiri et al. Diabetes 2001;50:2864-2869 ©2001 by American Diabetes Association