GPR124 is the receptor for WNT7B responsible for synergistic signaling

Slides:

Advertisements

Similar presentations

Productive Replication of Ebola Virus Is Regulated by the c-Abl1 Tyrosine Kinase by Mayra García, Arik Cooper, Wei Shi, William Bornmann, Ricardo Carrion,

Advertisements

Selective inhibition of BCL10-induced NF-κB activation by Tat–Peptide VIII HEK-293 cells were transiently co-transfected with an expression vector encoding.

The Long Non-Coding RNA XIST Interacted with MiR-124 to Modulate Bladder Cancer Growth, Invasion and Migration by Targeting Androgen Receptor (AR) Cell.

Epigallocatechin-3-Gallate Inhibits Matrix Metalloproteinase-9 and Monocyte Chemotactic Protein-1 Expression Through the 67-κDa Laminin Receptor and the.

Immuno-gold localisation of COPI in N. gruberi cells.

PKCK2 determines TRAIL sensitivity.

GLUT3 is a direct target of TCF4/β‐catenin

A. D. B. C. - ΔNP63α - β-Actin IMEC - SUM MDA-MB IMEC

A FOXO3a-BIM cascade mediates sensitivity to PARP and MEK inhibition

Supplemental Figure S1. Expression of MYOCD, MYOCD ceRNA and miRNAs

Volume 5, Issue 6, Pages (December 2013)

ABIN-1 inhibits IL-17–dependent NF-κB activation.

Supplementary Fig. S1 BCL2 5’flanking ctrl siRNA luc

XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells XIST regulated miR-29c by directly targetting in TMZ-resistant glioma cells.

Effect of Nf1 knockdown on Wnt pathway in MSC80 cells.

A and B, CO-1686–resistant NCI-H1975 cell clones, COR1-1 and COR10-1 display a reduced dependence on EGFR signaling for survival. A and B, CO-1686–resistant.

Knockdown efficiency of different shRNAs.

Fig. 5. Prip silencing enhances the co-localization of GABARAP with insulin vesicles and β-tubulin.Co-localization of GABARAP (green) with insulin (red)

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

NLK positively regulates YAP activity in physiological settings

TC-1 silencing sensitized A549 and SPC-A-1 cells to radiation therapy

Fig. 2. p85β regulates cell adhesion

Specific inhibition of Ras, but not Rac, inhibits EGF-stimulated protrusion. Specific inhibition of Ras, but not Rac, inhibits EGF-stimulated protrusion.

Fig. 4. Expression of p75NTR and time-course of TrkA autophosphorylation at the Y751 and Y490 sites in PC12-27 cells transfected with the vector, empty.

Fig. 3. Inactivation of the Wnt/β-catenin signaling pathway inhibited cell proliferation and induced apoptosis in A549 and SPC-A-1 cells. Inactivation.

Fig. 1. Loss of PC following INT depletion

The trafficking of VSVG–GFP to pre‐Golgi structures is impaired by depletion of GMAP‐210.The trafficking of VSVG–GFP to pre‐Golgi structures is impaired.

Abraxas binds to BRCT–PALB2(L21P) and is involved in the recruitment of this fusion protein or of endogenous PALB2 to sites of DNA damage. Abraxas binds.

Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 6. CBX7 expression in adipocyte differentiation of adipose-derived stem cells.(A) The adipose-derived stem cells, ADS1, were analyzed for the capability.

Cytoskeleton disruptors influence NMD or readthrough.

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

Expression of Wnt3EGFP in the cerebellum of Tg(wnt3:Wnt3EGFP)F3 partially compensates cerebellum growth in MO1-injected Wnt3 morphants. Expression of Wnt3EGFP.

Altered HIF-1α dynamics contribute to reduced VEGF expression in diabetic SIECs. (Aa–c) Total cell contents of HIF-1α, pAkt (at residue S473) and pS6 (at.

RXRa serves as a carrier for TR3 translocation initiated by 9-cis retinoic acid. RXRa serves as a carrier for TR3 translocation initiated by 9-cis retinoic.

Statistical chart of significantly differentially expressed genes

Fig. 4. Quantitative mRNA expression of two membrane-bound trehalase genes in Harmonia axyridis in response to starvation (0–72 h). Quantitative mRNA expression.

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.

Mechanism of Akt1 in promoting reprogramming.

Fig. 1. Loss of PC following INT depletion

Fig. 1. PARN is differentially expressed in breast cancer versus non-cancerous tissue/cells. PARN is differentially expressed in breast cancer versus non-cancerous.

Effect of GSK-3β inhibition on serum-starvation-induced apoptosis.

Effects of Chd1-knockdown on Hmgpi and Klf5 expression

WNT1 and WNT7B synergistically upregulate canonical Wnt target genes in YCC11 cells. WNT1 and WNT7B synergistically upregulate canonical Wnt target genes.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Synergistic WNT1 and WNT7B signaling is downstream of LRP6 phosphorylation and β-catenin stabilization. Synergistic WNT1 and WNT7B signaling is downstream.

CREB1 promotes the induction of endogenous ERα target genes.

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

miR-145 regulates embryo attachment.

The expression of two forms of N-cadherin.

Effect of the siRNA-mediated knockdown of endogenous MARCH8 on the expression levels of MARCH8 substrates, TfR and CD98, in HepG2 cells. Effect of the.

miR-145 overexpression reduces IGF1-coated bead attachment.

The mechanism of action for TRIO, CHKA and BMX lies between the AT1R and EGFR. (A) Our hypothesis is that TRIO, CHKA and BMX lie between the activated.

miR-145 regulates IGF1R expression.

Caveolin-1 downregulated survivin expression in cells with enhanced or constitutive activity via the Wnt pathway. Caveolin-1 downregulated survivin expression.

CO inhibits H2S generation through sGC-dependent cGMP production.

Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased survivin expression. Downregulation of caveolin-1 by si-RNA in NIH3T3 cells increased.

PALB2 is recruited by a pathway that mediates and recognizes protein ubiquitination at sites of DNA damage. PALB2 is recruited by a pathway that mediates.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

BCL-3 regulates expression of stemness-associated Wnt targets.

BCL-3 does not mediate β-catenin activity through promoting nuclear translocation or altering levels of LEF1. BCL-3 does not mediate β-catenin activity.

Inhibition of γ-secretase increased hair cell differentiation by upregulating Math1. a, Expression of Math1 in inner ear stem cells was increased after.

Effect of siRNA transfection on colony formation by Renca cells.

Role of Src and PTP-PEST in upregulation of tyrosine phosphorylation of paxillin and FAK. (A) Mock and D11 cells were pre-incubated with PP2 or PP3 (10 µM,

AP2μ2 has an essential function in Wnt/β-Catenin signaling.

Knockdown of ZBP1 in hippocampal neurons decreases dendritic branching

Effect of GSK-3β inhibition on cell-confluence-induced apoptosis.

Presentation transcript:

GPR124 is the receptor for WNT7B responsible for synergistic signaling. GPR124 is the receptor for WNT7B responsible for synergistic signaling. Knockdown of GPR124 in (A) HEK293-STF and (B) YCC11 cells with an siRNA pool (siGPR124; 100 nM) decreases the synergy from WNT1+WNT7B co-expression. siRNA was introduced 24 h prior to WNT transfection, and signaling was assessed 24 h later. Individual siRNAs had a similar effect (Fig. S2A). WNT1+WNT7B synergy was significantly reduced upon GPR124 knockdown (P<0.0001). (C) GPR124 knockdown inhibits synergy and WNT7B signaling as determined by measuring the amount of endogenous AXIN2. The experiments were performed as in B except AXIN2 was assessed by qRT-PCR and normalized to HPRT1. WNT7B signaling and WNT1+WNT7B synergy was significantly reduced upon GPR124 knockdown (P<0.0001). (D,E) Knockdown of RECK in (D) HEK293-STF and (E) YCC11 cells with an siRNA pool (siRECK; 100 nM) reduced WNT1+WNT7B synergy. WNTs were transfected 24 h post siRNA transfection and luciferase reporter activity was measured 24 h later. (F) Knockdown of RECK also inhibits WNT7B signaling and WNT1+WNT7B synergistic upregulation of the endogenous AXIN2. Graphs represent mean±s.d. (n=3). EV, empty vector; siC, control siRNA. Anshula Alok et al. J Cell Sci 2017;130:1532-1544 © 2017. Published by The Company of Biologists Ltd