Tsg protein stimulates endothelial cell migration, proliferation and collagen gel spheroid sprouting. Tsg protein stimulates endothelial cell migration,

Slides:

Advertisements

Similar presentations

G-Protein-Coupled Receptor 35 Mediates Human Saphenous Vein Vascular Smooth Muscle Cell Migration and Endothelial Cell Proliferation J Vasc Res 2015;52:

Advertisements

Fig. 3. ATME inhibits VEGF-induced invasion and tube formation of endothelial cells. (A) Effect of ATME on HUVEC invasion by using the Transwell culture.

From: Endogenic Regulation of Proliferation and Zinc Transporters by Pigment Epithelial Cells Nonvirally Transfected with PEDF Invest. Ophthalmol. Vis.

Mitochondria localize to both the front and the rear of neutrophils.

KG-501 suppresses NSCLC/IL-1β CM–induced migration of HUVECs by regulating IL-1β–induced CXC chemokine gene expression in NSCLC cells. KG-501 suppresses.

IL-1β significantly augments the angiogenic activity of NSCLC by inducing the expression of angiogenic CXC chemokine genes. IL-1β significantly augments.

Functional analysis of the cytoplasmic domain of the integrin α1 subunit in endothelial cells by Tristin D. Abair, Nada Bulus, Corina Borza, Munirathinam.

IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.

Bacterial lipopolysaccharide directly induces angiogenesis through TRAF6-mediated activation of NF-κB and c-Jun N-terminal kinase by Ingrid Pollet, Christy.

Sphingosine-1-phosphate promotes lymphangiogenesis by stimulating S1P1/Gi/PLC/Ca2+ signaling pathways by Chang Min Yoon, Bok Sil Hong, Hyung Geun Moon,

A Mechanism for Modulation of Cellular Responses to VEGF

Fig. 3. LTB4 exhibits concentration-dependent effects on HLEC lymphangiogenesis and survival. LTB4 exhibits concentration-dependent effects on HLEC lymphangiogenesis.

Induction of pro-angiogenic signaling by a synthetic peptide derived from the second intracellular loop of S1P3 (EDG3)‏ by Tamar Licht, Lilia Tsirulnikov,

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Ha Linh Vu, Sheera Rosenbaum, Claudia Capparelli, Timothy J

Fig. 3. Wisper controls CF behavior and survival.

PDGFRα GBM primary cultures demonstrate titratable and temporal DOX-inducible expression of hPDGF-A and receptor activation. PDGFRα GBM primary cultures.

Fig. 2. Circulating VEGF in GCA patients up-regulates microvascular endothelial Jagged1. Circulating VEGF in GCA patients up-regulates microvascular endothelial.

Testosterone dependent androgen receptor stabilization and activation of cell proliferation in primary human myometrial microvascular endothelial cells

Rena Watanabe et al. BTS 2016;1:

TRAF4 is required for EGFR activation in response to EGF stimulation.

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Mammary cell proliferation is inhibited when LAD1 is depleted.

Altered oligodendrocyte progenitor cell (OPC) marker protein expression in two white matter regions of ShhΔFP/ΔFP and SmoΔNT/ΔNT embryos at E15.5. Altered.

Fig. 4. A primary screen based on scrape closure

Fig. 3. Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr.

Fig. 4. Purified recombinant MRE11 from Thermotoga maritima removes topoisomerase IIα covalent complexes from genomic DNA.Purified recombinant MRE11 from.

Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.

Formation of papilloma lesions in the UroIICre+Fgfr3+/K644EK-RasG12D/+ model. Formation of papilloma lesions in theUroIICre+Fgfr3+/K644EK-RasG12D/+model.

IFN-γ and TNF-α cause an upregulation of Fas expression in human ESCs

Effects of doxorubicin and epirubicin on proliferation and apoptosis in the hyperplastic livers of transgenic zebrafish larvae. Effects of doxorubicin.

Fig. 5. Receptor tyrosine kinase activation in response to growth factor stimulation. Receptor tyrosine kinase activation in response to growth factor.

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Fig. 1. TSA at 165 nM induces maximal acetylation of histone H3 and near-maximal acetylation of histone H4.Immunoblot analysis of acetylated histones H3.

Fig. 5. Combination of SAHA and ML produces augmented suppression of cellular proliferation with impaired cell cycle progression, enhanced apoptotic.

Quantification and neutralization of VEGF in PDR vitreous.

Loss of LAR phosphatase activity is associated with decreased adhesion complex formation. Loss of LAR phosphatase activity is associated with decreased.

Macrophage-produced IL-10 is required but not sufficient for macrophage-stimulated proliferation of ADPKD cells. Macrophage-produced IL-10 is required.

The actin organization and N-cadherin dynamics in migrating cells.

V-ATPase and proteins involved in late endosomal protein sorting are required for efficient utilization of LDs during growth resumption. V-ATPase and proteins.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

VEGF stimulation together with inhibition of mTORC1 induces endothelial cell elongation independently of Foxo1. VEGF stimulation together with inhibition.

Ectopic Pax7 increases MyoD expression in Pax7-null myogenic cells.

Inhibition of Rho–ROCK–myosin switches from suppression to promotion of cell proliferation as matrix stiffness decreases. Inhibition of Rho–ROCK–myosin.

Proliferation on soft matrices is decoupled from myosin light chain phosphorylation, cell tractions, and focal adhesions. Proliferation on soft matrices.

The influence of SHIP2 on cell migration in breast cancer cells.

Fig. 1. Rnd2 and Rnd3 induce stress fibres whereas Rnd1 reduces stress fibres in endothelial cells.(A) Rnd mRNAs are expressed in HUVECs. Total RNA was.

Fig. 6. RhoB is required for Rnd3-induced stress fibre formation

Biphasic increase of PI(4,5)P2 level in LPS-stimulated cells.

Clustering of CD14 in the plane of the plasma membrane of LPS-stimulated cells. Clustering of CD14 in the plane of the plasma membrane of LPS-stimulated.

Depletion of COPI protein inhibits cis to trans cisternal maturation.

Effect of PDGF-BB on mesenchymal mesonephric cell migration.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

FAK is required for LPA-mediated induction of trailing-edge retraction and the restoration of normal morphology. FAK is required for LPA-mediated induction.

Dynamics of actin-binding proteins in the ICAM-1 complex.

Lack of Ctip2 is associated with impaired proliferation and delayed onset of differentiation during early stages of epidermal development. Lack of Ctip2.

Aplf downregulation does not induce cellular arrest.

Extracellular BMP modulators have different effects on endothelial cell sprouting in the tube formation assay. Extracellular BMP modulators have different.

Downregulation of Rab7 expression with RNAi caused accumulation of late autophagic vacuoles. Downregulation of Rab7 expression with RNAi caused accumulation.

Phenotype of S. pombe cells in the presence of B21P2 or LMB

Subcellular distribution of Mst4 and aPKCι in in vivo enterocytes.

Exosomal transfer of sortilin to a target cell.

Ctip2 directly regulates Notch1 expression in differentiated cells.

CCG regulates cellular localization and blocks target gene expression of MRTF. A, SK-Mel-147 cells were cotransfected with RhoC and the SRE.L reporter.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

FBXL19-AS1 knockdown inhibits proliferation, migration and invasion, and reduces the expression levels of angiogenesis related proteins in lung cancer.

Effect of IS on proliferation, senescence, and the production of NO and ROS from endothelial cells. Effect of IS on proliferation, senescence, and the.

VEGF165 stimulates migration of HMVECs

Effect of bevacizumab on the proliferation of A2780 cells.

Fig. 2 ITGA5 knockdown attenuates hPSC activation, differentiation, and functions. ITGA5 knockdown attenuates hPSC activation, differentiation, and functions.

Presentation transcript:

Tsg protein stimulates endothelial cell migration, proliferation and collagen gel spheroid sprouting. Tsg protein stimulates endothelial cell migration, proliferation and collagen gel spheroid sprouting. (A) For transmigration experiments HUVECs were serum-starved overnight and assayed with Tsg at the indicated concentrations or VEGF (100 ng/ml final concentration) as positive control, in migration medium containing 0.5% FBS. Triplicate samples were fixed after 4 hours and measurements were made on five random microscopic fields. Values are means ± s.e.m.; n = 3; *P<0.05 versus control. Hpf, high power field. (B) Proliferation was determined by a BrdU assay. Triplicate samples of HUVECs were incubated for 24 hours with BrdU and increasing concentrations of Tsg, or VEGF (100 ng/ml final concentration) as positive control, in medium containing 1% FBS. Cells in medium with BrdU only served as a negative control. Values are means ± s.e.m.; n = 4; *P<0.01 versus control. (C–E) HUVEC spheroids were embedded in collagen gel containing 10% FBS and stimulated for 24 hours with or without Tsg at the indicated concentrations, or with VEGF (50 ng/ml final concentration) as positive control. Representative spheroids incubated without (C) or with Tsg (D) are shown. Scale bars: 200 µm. (E) Quantitative analysis of cumulative sprout length of spheroids. Values are means ± s.e.m.; n = 3; * = P<0.01 versus control. Jennifer Heinke et al. J Cell Sci 2013;126:3082-3094 © 2013. Published by The Company of Biologists Ltd