The TSDR of ATL cells exhibits Treg-like hypomethylated status or CD4+ conventional T cell–like methylated status. The TSDR of ATL cells exhibits Treg-like.

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The TSDR of ATL cells exhibits Treg-like hypomethylated status or CD4+ conventional T cell–like methylated status. The TSDR of ATL cells exhibits Treg-like hypomethylated status or CD4+ conventional T cell–like methylated status. Bisulfite sequencing was performed using genomic DNA from each sample. The frequency of the demethylation of CpG is shown. Yellow, hypomethylated; and blue, methylated in each CpG. A, the DNA methylation status in the TSDR of the HTLV-1–infected cell lines and primary ATL cells was analyzed, and all TSDR, from amplicons (amp) 1 to 11, are illustrated. MJ cell line was used as the TSDR-hypomethylated representative, and MT-1 cell line was used as the TSDR-methylated representative. For primary ATL cells, case 2 was used as the TSDR-hypomethylated representative, and case 17 was used as the TSDR-methylated representative. B, the DNA methylation status of the TSDR of a healthy donor and primary ATL cells. Tregs from a healthy donor were used as the TSDR-hypomethylated control, and CD4+CD25− conventional T cells from a healthy donor were used as the TSDR-methylated control. The number of primary ATL cells corresponds to each case number shown in Supplementary Table S1. The data shown are the sum of three independent experiments. Yayoi Shimazu et al. Cancer Immunol Res 2016;4:136-145 ©2016 by American Association for Cancer Research