Ultrathin cryosections of 24-, 48-, and 66-h cysts, doubly labeled for 8C5 (5-nm Au) and TSA 417 (10-nm Au). Ultrathin cryosections of 24-, 48-, and 66-h.

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Ultrathin cryosections of 24-, 48-, and 66-h cysts, doubly labeled for 8C5 (5-nm Au) and TSA 417 (10-nm Au). Ultrathin cryosections of 24-, 48-, and 66-h cysts, doubly labeled for 8C5 (5-nm Au) and TSA 417 (10-nm Au). (A) In many 24-h encysting cells, 8C5 is localized to the cyst wall (cw), which has been deposited over the cell membrane (cm), which is decorated with TSA 417. 8C5 and TSA are both found in peripheral vacuoles (pv). The amount of TSA 417 on the cell membrane seems somewhat reduced. (B) In 48-h cysts, the cyst wall (cw) containing 8C5 has markedly increased in thickness and TSA 417 is completely absent from the cell membrane (cm). Note the small transport vesicles (small arrows). (C and D) In 66-h water-resistant cysts, TSA 417 is localized exclusively to the peripheral vacuoles and large internal vesicles and vacuoles, resembling the endosomal and prelysosomal compartments of higher eukaryotes. 8C5 is also present in many of these vesicles. Bars, 0.1 μm. Reprinted from reference 118 with permission of the publisher. Gaétan Faubert Clin. Microbiol. Rev. 2000; doi:10.1128/CMR.13.1.35