A Model for the Solution Structure of the Rod Arrestin Tetramer

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Presentation transcript:

A Model for the Solution Structure of the Rod Arrestin Tetramer Susan M. Hanson, Eric S. Dawson, Derek J. Francis, Ned Van Eps, Candice S. Klug, Wayne L. Hubbell, Jens Meiler, Vsevolod V. Gurevich  Structure  Volume 16, Issue 6, Pages 924-934 (June 2008) DOI: 10.1016/j.str.2008.03.006 Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 1 The Crystal Monomer and Location of Sites Studied The 2.8 Å crystal structure of the visual arrestin monomer (PDB ID: 1CF1, chain A) (Hirsch et al., 1999) with the C-tail colored light blue and the sites used for modeling shown in yellow. Cα and Cβ carbons indicate the direction in which the side chain projects. Structure 2008 16, 924-934DOI: (10.1016/j.str.2008.03.006) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 2 Flowchart of Molecular Modeling Steps (A) Low-resolution global search and data filter for dimer model generation. (B) Low-resolution global search and filters for tetramer model construction. (C) Building procedure and higher-resolution local search for tetramer model refinement. (D) Clustering for final model selection and refinement. Flowchart shapes indicate type of action performed: parallelogram (structure input/output); oval (model building operation); rectangle (conformational search algorithm); diamond (data filter or clustering decision point). DOF, degrees of freedom. Structure 2008 16, 924-934DOI: (10.1016/j.str.2008.03.006) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 3 Representative Models of the Arrestin Tetramer (A) Representative structures illustrating the breadth of search space covered in this study. Each tetramer is color coded by monomer. (B) Model 1 is a representative structure from the largest cluster, which was identified as the best structural model of the solution tetramer based on the criteria in Table 1. (C) A close-up view of model 1 showing the CC interface between two C domains (left) and the NN interface between two N domains (right). Residues shown to be located inside or outside contact interfaces (Hanson et al., 2007c) are highlighted in red and light blue, respectively. Structure 2008 16, 924-934DOI: (10.1016/j.str.2008.03.006) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 4 Concentration-Dependent Changes in the EPR Spectra of Spin-Labeled Arrestins Is Consistent with the Model (A) The spectrum of 10 μM S344R1 in the absence (black trace) and presence of 180 μM WT arrestin (red trace). (B) Close-up view of the CC and NN interfaces in model 1. Residues shown previously to be at or near a contact interface of the arrestin tetramer based on concentration-dependent changes in their EPR spectra (Hanson et al., 2007c) are shown in light blue and red (site 344). (C) Close-up view of model 1 NN interface, depicting residues 75 (red), and 376 and 381 (light blue). (D) For each site, the spectrum was recorded for 10 μM spin-labeled arrestin (black trace) and in the presence of 180 μM WT arrestin (red trace). For M75R1, the inset shows a magnified view of the low field region to more clearly reveal the immobilized component (arrow). The spectra are normalized to represent the same number of spins for each overlaid pair. Structure 2008 16, 924-934DOI: (10.1016/j.str.2008.03.006) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 5 Residues in Close Proximity in the Model Form Disulfide Bonds (A) Purified single cysteine mutants of arrestin in the presence (+) and absence (−) of 5 mM DTT were subjected to nonreducing SDS-PAGE and visualized by Coomassie staining as described in Methods. Molecular weight bands corresponding to arrestin dimer are present for 157C, 162C, 197C, and 348C in the absence of DTT. The apparent shift in molecular weight of the 197C dimer could be due to a difference in shape of the crosslinked species. (B) Close-up view of the NN and CC interfaces of model 1 highlighting the sites used for disulfide crosslinking. Residues that do and do not self-crosslink are shown in green and light blue, respectively. Structure 2008 16, 924-934DOI: (10.1016/j.str.2008.03.006) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 6 Removal of the C-Tail Enhances the Flexibility of the “Finger” Loop and Reduces Self-Association (A) The average molecular weight of Tr (1-378) arrestin and V74R1 on the background of full-length (74R1) and truncated (74R1Tr) as a function of total arrestin concentration (symbols) were determined from the light-scattering data and fit to the MDT model (solid lines) as described (Hanson et al., 2007c). The WT arrestin data (red X) are shown for comparison. (B) Internitroxide distance measurements of V74R1 on the background of full-length (green) and truncated (V74R1Tr) (black) arrestin by DEER spectroscopy. The DEER experiment measures the magnetic dipolar interaction between nitroxides as a modulation of the electron spin echo decay. The primary data is the echo amplitude as a function of time. The dipolar evolution function (left) is obtained after subtraction of an exponentially decaying background due to spins with randomly distributed interspin distances. Fourier transformation of the dipolar evolution function gives the Pake pattern (center). Fitting of the dipolar evolution function (left; red line) yields the experimental interspin distance distribution (right). Structure 2008 16, 924-934DOI: (10.1016/j.str.2008.03.006) Copyright © 2008 Elsevier Ltd Terms and Conditions

Figure 7 Targeted Disruption of Arrestin Self-Association (A) Close-up view of model 1 depicting the CC and NN interfaces. Residues 197, 267 (CC), and 85 (NN) are highlighted. (B and C) The average molecular weight of the indicated spin-labeled (R1) and alanine (A) arrestin mutants as a function of total arrestin concentration (symbols) were determined from the light-scattering data and fit to the MDT model (solid lines) as described (Hanson et al., 2007c). The WT arrestin data (red, X) are shown for comparison. Structure 2008 16, 924-934DOI: (10.1016/j.str.2008.03.006) Copyright © 2008 Elsevier Ltd Terms and Conditions