Subcellular distribution of Mst4 and aPKCι in in vivo enterocytes.

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Invest. Ophthalmol. Vis. Sci ;52(8): doi: /iovs Figure Legend:

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Subcellular distribution of Mst4 and aPKCι in in vivo enterocytes. Subcellular distribution of Mst4 and aPKCι in in vivo enterocytes. (A) Line plots showing the relative distribution of Mst4 versus actin along the apical–basal axis of in vivo enterocytes. (B) Double labeling for Mst4 (1,3; in 3, Mst4 is pseudo-colored magenta) and Rab11a (2,4). Overlay image is shown in panel 5, where white color indicates colocalization. Positive (+) PDM image representing quantitative analysis of colocalization is shown in panel 6. (C,D) Enlargements of the boxes indicated in B, panel 5 and indicated fluorescence intensity plots of Mst4 and Rab11a. (E) Line plots showing the relative distribution of aPKCι versus actin along the apical–basal axis of in vivo enterocytes. (F) Double labeling for aPKCι (1,3; in 3, aPKCι is pseudo-colored magenta) and Rab11a (2,4). Overlay image is shown in panel 5, where white color indicates colocalization. Positive (+) PDM image is shown in panel 6. (G,H) Enlargements of the boxes indicated in F, panel 5 and indicated fluorescence intensity plots of aPKCι and Rab11a. Asterisks indicate the position of the intestinal lumen. Scale bars: 10 µm. Herschel S. Dhekne et al. J Cell Sci 2014;127:1007-1017 © 2014. Published by The Company of Biologists Ltd