Engineering Robust Production Microbes for Large-Scale Cultivation

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Engineering Robust Production Microbes for Large-Scale Cultivation Maren Wehrs, Deepti Tanjore, Thomas Eng, Jeff Lievense, Todd R. Pray, Aindrila Mukhopadhyay Trends in Microbiology Volume 27, Issue 6, Pages 524-537 (June 2019) DOI: 10.1016/j.tim.2019.01.006 Copyright © 2019 Elsevier Ltd Terms and Conditions

Figure 1 Workflow from Proof-of-Principle Strain Development to an Optimized Conversion System for Industrial Scale-up. Strains exhibiting the desired phenotype at laboratory scale can be further examined through −omics and other analytical approaches to compare biological states at higher scales. Host selection, pathway optimization, and process optimization can and should be geared towards titer, yield, and rates, and also robustness in commercial-scale performance. Trends in Microbiology 2019 27, 524-537DOI: (10.1016/j.tim.2019.01.006) Copyright © 2019 Elsevier Ltd Terms and Conditions

Figure 2 Measurements during Strain Development in Microtiter Well Plates, Shake-Flasks, and Bioreactors. The measurements are not only different, but they also vary in availability. Whereas high-throughput studies at laboratory scale, such as in microtiter well plates (e.g., flower plates from BioLector Pro®) and shake-flasks, enable testing of a large number of conditions and strains in parallel, bioreactors offer an opportunity for time-series testing but at very low relative throughput to compare strains and operating conditions. DCW, dry cell weight; OD, optical density; WCW, wet cell weight. Trends in Microbiology 2019 27, 524-537DOI: (10.1016/j.tim.2019.01.006) Copyright © 2019 Elsevier Ltd Terms and Conditions