Co-expression of VSVG–Cdc42-CA, but not VSVG–Cdc42-DN with eGFP–gephyrin (green) and Myc–CB2ΔPH (blue) rescues postsynaptic gephyrin clustering, as shown.

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Presentation transcript:

Co-expression of VSVG–Cdc42-CA, but not VSVG–Cdc42-DN with eGFP–gephyrin (green) and Myc–CB2ΔPH (blue) rescues postsynaptic gephyrin clustering, as shown by immunofluorescence staining of triple-transfected neurons (11+7 DIV) with synapsin-1 (red). Co-expression of VSVG–Cdc42-CA, but not VSVG–Cdc42-DN with eGFP–gephyrin (green) and Myc–CB2ΔPH (blue) rescues postsynaptic gephyrin clustering, as shown by immunofluorescence staining of triple-transfected neurons (11+7 DIV) with synapsin-1 (red). (A) Myc–CB2ΔPH with no Cdc42. (A′) Postsynaptic gephyrin clusters are seen in dendrites (arrowheads) and on the soma upon the co-transfection of VSVG–Cdc42-CA. (A″) Gephyrin streaks and intrasomatic aggregates are readily evident upon co-transfection of VSVG–Cdc42-DN. Similar results were obtained in three independent experiments. Scale bar: 20 μm. Shiva K. Tyagarajan et al. J Cell Sci 2011;124:2786-2796 © 2011.