Inducible liver-specific insulin receptor knockout (iLIRKO) shows insulin resistance in the liver and extrahepatic tissues. Inducible liver-specific insulin.

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Inducible liver-specific insulin receptor knockout (iLIRKO) shows insulin resistance in the liver and extrahepatic tissues. Inducible liver-specific insulin receptor knockout (iLIRKO) shows insulin resistance in the liver and extrahepatic tissues. In vivo insulin signaling studies were performed in 6-month-old control and iLIRKO mice. Mice of both experimental groups were injected with 1 unit/kg body wt of human insulin (Novo Nordisk) into the peritoneal cavity. After 10 min of treatment, liver, muscle, BAT (brown adipose tissue), and brain were removed. Protein extracts of these tissues were analyzed by Western blot (WB) with anti-IR β-chain, phospho-Akt, phospho-p70, phospho-ERK1/2, and β-actin antibodies. The results are representative of four independent experiments. Histograms summarize the densitometric analysis of the corresponding Western blots. Oscar Escribano et al. Diabetes 2009;58:820-828 ©2009 by American Diabetes Association