A statistic for microtubule order in mitotic spindles.

Slides:

Advertisements

Similar presentations

Immuno-gold localisation of COPI in N. gruberi cells.

Advertisements

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Differences in cellular organization emerge from quantitative proteomic experiments Differences in cellular organization emerge from quantitative proteomic.

Topographical effects of cell line, mitotic phase, and inhibitors

Qiannan Wang, Shanjin Huang Molecular Plant

Leukemia involves the accumulation of ISP CD8+T-cells and blockages in B-cell development. Leukemia involves the accumulation of ISP CD8+T-cells and blockages.

Multiple Conformations of F-actin

Qiannan Wang, Shanjin Huang Molecular Plant

Robust Driving Forces for Transmembrane Helix Packing

Fig. 7. Motion adaptation increases time-dependent response modulations (TDRM) relatively to the average cell response.TDRM normalized to the value obtained.

Fig. 2. Proportion of motile objects and track length

Fig. 6. Comparison between the response against transformed tissues and capsule formation.At the cellular level the two responses share many similarities.

Concentration of biomarkers of haemostasis.

Positive relationship between the residual breaking force and cuticle area of lamellar joints. Positive relationship between the residual breaking force.

Fig. 1. Morphological and growth characterization of hBMCs and hPDCs

Fig. 4. The model of malate metabolism in fruit cells under different K level conditions. The model of malate metabolism in fruit cells under different.

Fig. 1. E-cadherin localizes in nano-scale clusters.

Fig. 4. Acentriolar mitotic spindle assembly

Fig. 5. Recovery steps of mitotic acentriolar cells after cold MT depolymerisation.(A,B) Time-lapse sequences of mitotic spindle re-formation at 18°C.

Image cross-correlation analysis reveals the emergence of a dynamic steady state actin distribution in the minimal cortex. Image cross-correlation analysis.

Proteins associated with PfSec13.

Fig. 6. Comparison of Plk4 with Sas-6 localization

Fig. 2. Morphological analysis of acentriolar mitotic spindles

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 2. Centrosomal proteins display distinct localizations and radial distances from centriole walls.U2OS cells were fixed and stained with the indicated.

Fig. 2. Effect of substrate orientation on growth rate of midwater tadpoles with different oral configurations. Effect of substrate orientation on growth.

Transcription factor network of human pancreas development.

Integrin binding of soluble GRGDSP and fibronectin.

Fig. 4. Co-immunostaining of nocodazole or ASNase treated RPE-1 cells with anti-hASNS and anti-alpha tubulin showed defect in both mitotic spindle formation.

The interaction of gecko toe pads with the substrate in the presence and absence of water. The interaction of gecko toe pads with the substrate in the.

Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

Body density decreased and buoyancy increased as air sac volume increased with inflation pressure in the three species. Body density decreased and buoyancy.

Effects of the ft-overexpressing clones in A and P compartments (cf.

Fig. 3. Effect of substrate orientation on growth rate for bottom-dwelling tadpoles with similar oral configuration. Effect of substrate orientation on.

BMP signalling is dispensable for early gastruloid patterning.

Fig. 5. Combination of SAHA and ML produces augmented suppression of cellular proliferation with impaired cell cycle progression, enhanced apoptotic.

Comparison ofMyc-induced zebrafish liver tumors with different stages of human HCC and seven mouse HCC models. Comparison ofMyc-induced zebrafish liver.

FAK/Src activity is required to trap PTP1BDA in adhesions.

Fig. 8. The morphology of the ventral nerve cord in Ror-Myc overexpressing embryos is normal. The morphology of the ventral nerve cord in Ror-Myc overexpressing.

Statistical chart of significantly differentially expressed genes

BAF60c transcriptionally affects cardiac morphogenesis and function

Example of a putative bridging fiber.

SBF-SEM of mitotic spindles.

Tau–RFP–RBPs colocalize with mRNA on microtubules and lead to the wetting of stress granules on microtubules. Tau–RFP–RBPs colocalize with mRNA on microtubules.

Volume 94, Issue 7, Pages (April 2008)

Single MTs can be resolved in subsections of SBF-SEM image stacks.

Localization and cellular function of spectraplakins.

Subdiffraction-limit imaging of BRCA1 and 53BP1 in IRIF

Spatial statistics of X-ray volumes reveal layering and spatially diverse distribution of cell bodies. Spatial statistics of X-ray volumes reveal layering.

Ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis. ibm1 and edm2 mutants generate more stomatal divisions in the leaf epidermis.

dcn1-deletion results in attenuated cohesin cleavage at anaphase

HIV Broadly Neutralizing Antibodies: Taking Good Care Of The 98%

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

KIF13A overexpression favors NP distribution to the cell edge and requires KIF13A binding to microtubules. KIF13A overexpressionfavorsNP distribution to.

miR-145 overexpression reduces IGF1-coated bead attachment.

Kinematic measurements recorded as squid approached shrimp and fish.

Altered intracellular distributions of acetylated α-tubulin, mitochondria and peroxisomes. Altered intracellular distributions of acetylated α-tubulin,

Peroxisome speeds were slower in patient and control cells.

Fig. 5. Testis defects in STK35 KO mice.

Nutrient availability regulates growth of the X. laevis optic tectum.

Kinetics of BDNF-induced Erk, Akt and PLCγ activation in the presence of 15 mM NaCl or 15 mM KCl. Representative western blots (A) and quantitative plots.

The expression pattern of marker proteins for sensory neuron subgroups changes during postnatal maturation. The expression pattern of marker proteins for.

Sensory neurons grow and downregulate UCHL1 expression levels during postnatal maturation. Sensory neurons grow and downregulate UCHL1 expression levels.

Tension develops across sister kinetochores (red) upon their bipolar attachment by spindle microtubules (black line). Tension develops across sister kinetochores.

Isolation of large and small prominin-1-containing membrane particles from the embryonic ventricular fluid. Isolation of large and small prominin-1-containing.

Membrane integration of the model constructs depends on protein sequence, not codon usage. Membrane integration of the model constructs depends on protein.

Structural insights based on chimeric Alp4-GCP2 analysis.

Example of swimming trajectories from three groups of fish from three different weeks along with the extracted behavioral parameters. Example of swimming.

Subcellular distribution of Mst4 and aPKCι in in vivo enterocytes.

ASPP2 hydroxylation is detectable in vivo and the amount of hydroxylation depends on FIH-1 abundance. ASPP2 hydroxylation is detectable in vivo and the.

Presentation transcript:

A statistic for microtubule order in mitotic spindles. A statistic for microtubule order in mitotic spindles. (A) Two examples of angle analysis. (i) MTs from a control cell and a cell overexpressing GFP–TACC3 are shown colored according to their deviation from the respective model spindle. In the right panel, MTs with deviations of less than 20° have been removed for clarity. The plots show a z-projection of the entire volume. (ii) Histogram of angle deviations for the spindles. Inset: comparison of the two histograms shown as a normalized probability density function. (B) Summary of cellular conditions analyzed under warm and cold conditions. (C) Heat map to show Π(Θ1, Θ2) for several conditions as indicated. Π is a comparison of angles from two data sets: values greater than 0.5 indicate that Θ2 MTs deviate more from the ideal data than those in Θ1 (see Materials and Methods, Model discrepancy and comparison for further details). n=5031–23240 segments from 1–4 cells. All datasets in this figure are shown in Fig. S1. Faye M. Nixon et al. J Cell Sci 2017;130:1845-1855 © 2017. Published by The Company of Biologists Ltd