Vps36 regulates the accumulation of Smo and Hh signaling activity.

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Vps36 regulates the accumulation of Smo and Hh signaling activity. Vps36 regulates the accumulation of Smo and Hh signaling activity. (A–F) Wing imaginal discs expressing the indicated genes in wing pouch driven by MS1096-Gal4 were stained with anti-lacZ (green) and anti-Smo (red) or anti-FLAG (red) antibodies to show the expression of dpp–lacZ (A,B,E,F) or ptc–lacZ (C,D), endogenous Smo (A–D) or FLAG-tagged Smo (FLAG–Smo) (E,F). White arrows indicate the ectopic expression of either dpp–lacZ or ptc–lacZ. Overexpression of Vps36 RNAi caused accumulation of Smo in the A compartment (compare Smo staining in A′ and B′, C′ and D′) and upregulates dpp expression (compare dpp–lacZ staining in A and B) and ptc expression (compare ptc–lacZ staining in C and D). Overexpression of FLAG–Smo driven by MS1096-Gal4 resulted in the upregulation of dpp expression (E). Knockdown of Vps36 further increased dpp expression (compare dpp–lacZ staining in E and F). In addition, the magnified images showed that knockdown of Vps36 results in Smo accumulation on the cell plasma membrane in the A compartment (compare Smo staining in A‴ and B‴). The white squares in A″ and B″ mark the magnified regions for A‴ and B‴, respectively. Arrows indicate the expression of dpp–lacZ (B,E,F) or ptc–lacZ (C,D), respectively. Scale bars: 5 µm (A‴ and B‴). Xiaofeng Yang et al. J Cell Sci 2013;126:4230-4238 © 2013. Published by The Company of Biologists Ltd