Effect of myosin Vb shRNA on ezrin phosphorylation and microvilli organization in Caco-2 cells. Effect of myosin Vb shRNA on ezrin phosphorylation and.

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Effect of myosin Vb shRNA on ezrin phosphorylation and microvilli organization in Caco-2 cells. Effect of myosin Vb shRNA on ezrin phosphorylation and microvilli organization in Caco-2 cells. (A,B) Immunofluorescence labeling of T567-phosphorylated ERM (large tilted x-y and top x-z image) and ezrin (bottom x-z image) in control (D) and myosin-Vb-knockdown (E) cells. GFP (green) labels transduced cells. Arrows and asterisks in x-y and x-z images, respectively, show the presence (A) or absence (B) of T567-phosphorylated ERM. (C) Expression of ezrin, T567-phosphorylated ezrin, actin and GAPDH. (D,E) Representative scanning electron micrographs of the apical surface of control and myosin-Vb-knockdown cells. (F) Semi-quantitative analysis of microvilli density per unit surface area, on a scale from ‘+++’ (very dense; see D for a representative +++ surface area) to ‘−’ (no microvilli; see E for a representative − surface area). Scale bars: 10 µm. Herschel S. Dhekne et al. J Cell Sci 2014;127:1007-1017 © 2014. Published by The Company of Biologists Ltd