Functional analysis of CAR-redirected primary NK cells.

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Presentation transcript:

Functional analysis of CAR-redirected primary NK cells. Functional analysis of CAR-redirected primary NK cells. A, Representative gating strategy and contour plots to illustrate functional analysis of bulk primary NK cells. B, Summary graphs of six to nine separate experiments (n = 6–12) showing degranulation response against CD19− (P815 and K562) and CD19+ (221.wt and NALM-6) targets in NK cells transfected with HA21 CAR construct. Statistically significant difference between mock- and CAR-transfected cells indicated by black asterisk with underline (n = 12–14). Summary graphs of two separate experiments with three donors (n = 6) showing NK-cell (C) degranulation response and (D) IFNγ+ NK cells against CD19− (P815 and K562) and CD19+ (221.wt and NALM-6) targets across different CAR constructs (n = 15). Summary graphs of nine separate experiments showing (E) degranulation response and (F) IFNγ+ NK cells against CD19− (K562) and CD19+ (221.wt and NALM-6) targets in NK cells transfected with either HA21 or 41BB/ζ CAR construct (n = 12). Statistically significant difference between mock- and CAR-transfected cells indicated by black asterisk with underline. G, Graph showing CAR expression following repeated target or control cells interactions. An arrow indicates when the new pool of target cells was added (n = 4). Experiments were performed three times. Vincent Yi Sheng Oei et al. Cancer Immunol Res 2018;6:467-480 ©2018 by American Association for Cancer Research