Fertility and Sterility

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Fertility and Sterility Biochemical and molecular studies of the proacrosin/acrosin system in patients with unexplained infertility Sara I Marí, M.Sc., Vanesa Rawe, Ph.D., Juan C Biancotti, M.Sc., Eduardo H Charreau, Ph.D., Liliana Dain, Ph.D., Mónica H Vazquez- Levin, Ph.D. Fertility and Sterility Volume 79, Pages 1676-1679 (June 2003) DOI: 10.1016/S0015-0282(03)00372-8

FIGURE 1 Analysis of human proacrosin activation in protein sperm extracts. Acid sperm extracts were incubated under proacrosin activation conditions for 0 (t0) and 60 (t60) minutes, and were analyzed by Western immunoblotting with antiacrosin antibody AcrC5F10. Proacrosin mature β-acrosin are indicated with arrows. Each sample was run at least two times. Protein patterns are shown of normal acrosin activity samples (lanes a, b, c, and d, from patients P4, P5, P6, and P13, respectively) and of abnormal low acrosin activity (lanes e, f, g, and h, from patients P9, P11, P16, and P25, respectively). Man. Acrosin in unexplained infertility. Fertil Steril 2003. Fertility and Sterility 2003 79, 1676-1679DOI: (10.1016/S0015-0282(03)00372-8)