Effect of GSK-3 inhibitors on basal and insulin-stimulated glucose uptake in human muscle cells. Effect of GSK-3 inhibitors on basal and insulin-stimulated.

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Philip J. Barter, et al. Circulation 2011;124:

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Effect of GSK-3 inhibitors on basal and insulin-stimulated glucose uptake in human muscle cells. Effect of GSK-3 inhibitors on basal and insulin-stimulated glucose uptake in human muscle cells. A: Time course of glucose uptake activation in human muscle cells by CHIR 98023 (2 μmol/l), insulin (33 nmol/l), or LiCl (20 mmol/l). Results are averages ± SE, normalized against glucose uptake in untreated control cells from the same individual (n = 4). B: Muscle cells from nondiabetic (n = 5) and type 2 diabetic (n = 4) subjects were treated for 4 days with CHIR 98023 (2.5 μmol/l), CHIR 98014 (2.5 μ mol/l), inactive compound (IA, 2.5 μmol/l), or LiCl (20 mmol/l), as described in detail in research design and methods. After chronic treatment, half of the cell cultures were washed with fresh media and incubated with 33 nmol/l insulin for 2 h. Results are averages ± SE, normalized against the control and basal value for each individual. *P < 0.05 vs. untreated paired control; †P < 0.05 vs. paired basal. Svetlana E. Nikoulina et al. Diabetes 2002;51:2190-2198 ©2002 by American Diabetes Association