Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional.

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Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. Effect of ERα Ub-binding ability on E2-induced receptor transcriptional activity. (A,C,D) Luciferase assay on the HeLa cells transiently transfected with pcDNA FLAG-ERα (wt) or the pcDNA FLAG-ERα (LAAG) mutant together with either the 3×ERE TATA reporter plasmid (A), with pXP2-D1-2966 cyclin D1 reporter plasmid (pD1) (C) or with the protein complement 3 (pC3) reporter plasmid (D) and then treated for 24 hours with different doses of E2 (A) or with 10-9 M E2 (C,D). (B) Western blot analysis of the Bcl-2 protein levels in the HeLa cells transiently transfected with pcDNA FLAG-ERα (wt) or the pcDNA FLAG-ERα (LAAG) mutant and then treated for 24 hours with different doses of E2. Loading control was done by evaluating vinculin expression in the same filter. * indicates significant differences with respect to the relative C sample; ° indicates significant differences with respect to the relative wt ERα E2-treated sample. Valeria Pesiri et al. J Cell Sci 2013;126:2577-2582 © 2013. Published by The Company of Biologists Ltd