Weight loss improves in vitro adipogenesis.

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Weight loss improves in vitro adipogenesis. Weight loss improves in vitro adipogenesis. Cells were differentiated for 12 days, and then accumulation of lipids, protein content, or gene expression was analyzed. A: Effect of weight loss on lipid accumulation. Representative images of adipocytes from one donor before and after DI stained with ORO and quantification of neutral lipid accumulation expressed as percent of stock ORO (n = 22). B: Effect of weight loss on gene expression. mRNA expression (arbitrary units [AU]) in adipocytes normalized to GUSB expression (n = 15). C: Effect of weight loss on protein content. Ratio between total protein content in adipocytes vs. preadipocytes (n = 22). Data are means ± SE; *P < 0.05, **P < 0.01, ***P < 0.001. D: Linear regression between ORO accumulation and protein content in adipocytes (A) vs. preadipocytes (P) at baseline. E: Linear regression between relative Δprotein (adipocytes vs. protein) and ΔmRNA expression of aP2 and PPARγ. Lenka Rossmeislová et al. Diabetes 2013;62:1990-1995 ©2013 by American Diabetes Association