UVA Radiation Induces Suppression of Perforin-Granule Release from CD8hi+ Cytotoxic T Lymphocytes Ex Vivo  Andreas Ambach, Bernd Bonnekoh, Harald Gollnick  Journal of Investigative Dermatology  Volume 123, Issue 1, Pages 237-239 (July 2004) DOI: 10.1111/j.0022-202X.2004.22705.x Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Ultraviolet (UV)A concentration-dependent inhibition of PMA/ionomycin-stimulated perforin-granule release from CD8hi+ cytotoxic T lymphocytes (CTL). Peripheral blood mononuclear cells were radiated with various doses of full spectrum UVA prior to PMA/ionomycin stimulation. The perforin-positive portion of CD8hi+ CTL was determined and set in relation to the unirradiated control at time zero. 10 J per cm2 UVA (n=6; C) was more effective than 5 J per cm2 (n=6; B) in delaying the PMA/ionomycin-stimulated perforin release, whereas 3 J per cm2 (n=6; A) and 30, 70, 100, or 150 UVB mJ per cm2 (n=6; D) did not influence this process. Statistical significance was calculated in relation to PMA/ionomycin-stimulated unirradiated cells (*p<0.05, **p< 0.01, ***p<0.001). Unirradiated, unstimulated control, ; PMA/ionomycin-stimulatory treatment, ; UV-radiated samples, dotted lines. Journal of Investigative Dermatology 2004 123, 237-239DOI: (10.1111/j.0022-202X.2004.22705.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions