Detection of GPR55 ligand in the small intestine.

Slides:

Advertisements

Similar presentations

Impaired development of Treg cell function upon overexpression of FOXP3A384T in naïve CD4+CD25− T cells. Impaired development of Treg cell function upon.

Advertisements

Specific depletion of TFH and LCMV-specific CD4 T cells.

MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific T-bet+ population. MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific.

CD8α+ DC-deficient mice are highly susceptible to Lm infection in the absence of CD169+ macrophages. CD8α+ DC-deficient mice are highly susceptible to.

Increased movement of GPR55 KO cells in association with epithelial cells. Increased movement of GPR55 KO cells in association with epithelial cells. (A)

Specific depletion of CD4-DTR–derived CD4 T cells.

Three different types of transfer functions with a codomain of [0,1].

Splenic CD169+ macrophages express a unique gene profile.

CD169+ macrophages mediate Lm translocation to the splenic T cell zones. CD169+ macrophages mediate Lm translocation to the splenic T cell zones. (A) Confocal.

Improvement in the transcriptional activity of FOXP3A384T by enhancement of TIP60-FOXP3 interaction. Improvement in the transcriptional activity of FOXP3A384T.

Workspace comparison of Delta robots.

Protein sequence alignment of the NS3 helicase–encoding region of 63 flaviviruses demonstrates conservation of a KIR2DS2-binding peptide. Protein sequence.

Increased resistance of GPR55-deficient mice to indomethacin-induced intestinal permeability. Increased resistance of GPR55-deficient mice to indomethacin-induced.

Altered distribution of γδT IELs in GPR55-deficient mice.

Human PBMC-derived MERS-CoV–specific T cells are multifunctional.

Virus-specific T cell responses are detected in all MERS survivors.

bnmAbs 3D3 and 2D10 specifically block RSV G –induced chemotaxis.

Comparison of repertoire distributions to baseline.

Summary of time-limited events in early life promoting tolerance to gut bacterial antigens. Summary of time-limited events in early life promoting tolerance.

Genetic FIP200 deletion impairs autophagy induction and causes T cell apoptosis. Genetic FIP200 deletion impairs autophagy induction and causes T cell.

β-Glucans do not modulate epithelial IL-33 or AHR.

Tfr cells robustly secrete IL-10 after acute viral infection.

DC subset cooperation for activation of antiviral T cells.

Neutrophil recruitment to the colonic lamina propria depends on CD4+ T cells. Neutrophil recruitment to the colonic lamina propria depends on CD4+ T cells.

Definition of the cellular source of IL-22.

TCRs bind to CD1b-PG complexes formed in cells.

T-bethi MP cells produce IFN-γ in response to IL-12.

TCR signaling is required for exhausted-like TRM cell formation and maintenance. TCR signaling is required for exhausted-like TRM cell formation and maintenance.

Protein sequence alignment of the NS3 helicase–encoding region of 63 flaviviruses demonstrates conservation of a KIR2DS2-binding peptide. Protein sequence.

BAP1 deficiency results in thymic atrophy and loss of thymocyte populations. BAP1 deficiency results in thymic atrophy and loss of thymocyte populations.

Neutrophil depletion ameliorates colitis in Cx3cr1cre:Il10rafl/fl BM chimeras. Neutrophil depletion ameliorates colitis in Cx3cr1cre:Il10rafl/fl BM chimeras.

Deubiquitinating activity, but not HCF-1 binding, is required for BAP1 function in thymocytes. Deubiquitinating activity, but not HCF-1 binding, is required.

The microbiota inhibits colonic GAPs and antigen delivery after weaning. The microbiota inhibits colonic GAPs and antigen delivery after weaning. (A) Expression.

Inhibiting or altering the timing of microbial antigen encounter results in inflammatory T cell responses against gut bacteria. Inhibiting or altering.

GPR55 restrains IEL accumulation in the small intestine.

Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.

A summary of different energy sources for robotics.

Microrobots with different cell-carrying capacities under different grid lengths (lg) and burr lengths (lb). Microrobots with different cell-carrying capacities.

Ligands eluted from hpMR1+EC and hpMR1+MS contain both shared and unique ions. Ligands eluted from hpMR1+EC and hpMR1+MS contain both shared and unique.

MR1T clones with distinct TCR usage display differential recognition of discrete activating ligands. MR1T clones with distinct TCR usage display differential.

PLI and PLIII are activating ligands for MR1Ts.

Shared phenotype of CD4+CLA+CD103+ T cells from human blood and skin.

T cell–derived cytokines drive immune evasion.

Blood Tfr cells do not show specialized humoral regulatory capacity.

MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. PBMCs.

RORα deficiency in Tregs results in exaggerated skin inflammation in response to EC sensitization. RORα deficiency in Tregs results in exaggerated skin.

CD25 surface expression and TCR signal strength predict T helper differentiation and memory potential of early effector T cells in vivo. CD25 surface expression.

BMS blocks functional responses in primary immune cells driven by IFNα

Fig. 6 SNP rs is a functional SNP in 22Rv1 cells.

Fig. 4 Reconstitution of MCs in KitW-sh/W-sh mice increases IL-10+ Breg cells and suppresses the CHS response. Reconstitution of MCs in KitW-sh/W-sh mice.

Tfr cells respond better to immunization with self-antigens than with foreign antigens. Tfr cells respond better to immunization with self-antigens than.

Inhibiting or altering the timing of microbial antigen encounter results in inflammatory T cell responses against gut bacteria and worsened colitis upon.

GPR55 mediates lymphocyte migration inhibition via Gα13.

AEGIS autonomous targeting process.

Partial alteration of the Treg gene signature by the p.A384T mutation.

Fig. 3 Local Maraba treatment of TNBC tumors provides long-term systemic protection. Local Maraba treatment of TNBC tumors provides long-term systemic.

Colonic Treg TCRs react to MA Helicobacter species.

GPR55 regulates γδT cell egress from PP and homing of gut-tropic CD8 T cells to the small intestine. GPR55 regulates γδT cell egress from PP and homing.

Bacterial antigen encounter occurs during a specific preweaning interval, is dependent on GCs, and correlates with the presence of colonic GAPs. Bacterial.

miR-146a is highly expressed selectively on γδ27− T cells.

Lin28b promotes the positive selection of CD5+ ImmB cells in neonatal mice. Lin28b promotes the positive selection of CD5+ ImmB cells in neonatal mice.

Acute circadian disruption alters ILC3 cytokine secretion.

REV-ERBα deficiency reduces frequency and number of NKp46+ ILC3s.

Circadian gene expression in ILC3s is associated with rhythmic cytokine expression. Circadian gene expression in ILC3s is associated with rhythmic cytokine.

Meningeal γδ T cells are biased toward IL-17 production.

Fetal-derived γδ T cells infiltrate the meninges from birth.

In response to allergen, T cells and ILCs are equally important sources of IL-13. In response to allergen, T cells and ILCs are equally important sources.

Bb monocolonization enhances Treg population in the cLP.

CCR6 is dispensable for expansion of innate TCRαβ+ cells in oral candidiasis. CCR6 is dispensable for expansion of innate TCRαβ+ cells in oral candidiasis.

CSGG-induced iTreg cells are capable of suppressing intestinal inflammation. CSGG-induced iTreg cells are capable of suppressing intestinal inflammation.

Presentation transcript:

Detection of GPR55 ligand in the small intestine. Detection of GPR55 ligand in the small intestine. (A) Abundance of the indicated forms of LPI in spleen (n = 5) and small intestine (n = 5) tissue determined by LC-MS/MS. (B and C) Bioassay measurement of relative GPR55 ligand activity in small intestine, colon, and spleen extract (B) and in small intestinal epithelial cell (SI-EC) culture supernatants (C). WEHI231 cells transduced with GPR55 were tested for their response to the tissue extracts in a Transwell migration assay. Extract at the indicated dilution was mixed with CXCL12 (100 ng/ml). Each experiment was performed in duplicate and repeated three times. (D) Histograms showing Thy1.1 reporter expression on intestinal and spleen DCs in mice that had been reconstituted with empty vector– or GPR55-transduced BM. (E to G) Summary data from mice of the type in (D), shown as the ratio of Thy1.1+/Thy1.1− cells of the indicated types within the intestine (E and F) and spleen (G). n = 4 in each group. **P < 0.01, *P < 0.05, n.s. P > 0.05 by Student’s t test. Hayakazu Sumida et al. Sci. Immunol. 2017;2:eaao1135 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works