APLF regulates genes implicated in MET during the generation of iPSCs from fibroblasts. APLF regulates genes implicated in MET during the generation of.

Slides:

Advertisements

Similar presentations

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

Advertisements

Volume 2, Issue 5, Pages (May 2014)

Scanning electron microscopy analysis of EGK-I to -V chick embryos.

Volume 9, Issue 5, Pages (November 2017)

Volume 5, Issue 3, Pages (September 2015)

Endogenous SRP19 is localized to the nucleus and the nucleolus.

Fig. 3. Wisper controls CF behavior and survival.

Fig. 5. Stimulation of EPO expression and secretion under hypoxic culture conditions. Stimulation of EPO expression and secretion under hypoxic culture.

Volume 3, Issue 5, Pages (November 2014)

Yifang Chen, Devendra S. Mistry, George L. Sen

Volume 9, Issue 5, Pages (November 2017)

Change in mRNA expression of Glasso network genes in differentiated PC12 cells following Caprin2 knockdown or Caprin2 overexpression. Change in mRNA expression.

QRT-PCR analysis of the role of MDL-1 in expression of infection-regulated genes. qRT-PCR analysis was performed on infection-regulated genes that have.

Expression of Glasso network genes in PC12 cells.

AKT2, but not AKT1, is required for regulating survival of PTEN-deficient prostate tumor spheroids. AKT2, but not AKT1, is required for regulating survival.

MAPK14 regulates ROS generation in GS cells.

BTN1 mRNA level in response to extracellular pH determined by comparative RT2PCR. BTN1mRNA level in response to extracellular pH determined by comparative.

Fig. 2. Morphological changes of cultured adherent fibroblastic cells after OA treatment related to actin microfilament reorganization.(A) Cells observed.

Altered oligodendrocyte progenitor cell (OPC) marker protein expression in two white matter regions of ShhΔFP/ΔFP and SmoΔNT/ΔNT embryos at E15.5. Altered.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

Fig. 1. Lack of Hmga1 and Hmga2 expression in A1/A2-KO mice

Fig. 4. Increased adipocyte differentiation in Cbx7-KO ES cells

Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.

Fig. 7. E2F1 acetylation in A1/A2-KO MEFs

Effect of Z-FA-FMK on the expression of SMN proteins in SMA patient iPSCs and iPSC-derived motor neurons. Effect of Z-FA-FMK on the expression of SMN proteins.

Fig. 3. Coilin levels correlate with altered nascent U2 snRNA, hTR and rRNA levels.RNA isolated from HeLa or WI-38 cells following RNAi targeting coilin.

Identification of Z-FA-FMK as a potent compound that increases SMN protein expression in HEK293 and patient fibroblast cells. Identification of Z-FA-FMK.

High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. High-affinity binding to the.

Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

HSP90β interacts with HNF4A to regulate protein half-life.

An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic progenitor cells. An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic.

Fig. 4. Quantitative mRNA expression of two membrane-bound trehalase genes in Harmonia axyridis in response to starvation (0–72 h). Quantitative mRNA expression.

Ang-1, ESE-1 and Tie-2 expression levels during teratogen-induced CDH

Fig. 1. Phenotypic characterisation of primary human tubular epithelial cells and human renal fibroblasts. Phenotypic characterisation of primary human.

Mechanism of Akt1 in promoting reprogramming.

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

PTK6 downregulation suppresses SNAIL and increases E-cadherin expression. PTK6 downregulation suppresses SNAIL and increases E-cadherin expression. A,

Effects of Z-FA-FMK on SMN protein expression in type II SMA patient fibroblast cells (GM22592). Effects of Z-FA-FMK on SMN protein expression in type.

Fig. 1. Optogenetic activation of EphB2.

N-cadherin deficient cells do not adhere or migrate.

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. Fgfr3;4 mutant lungs display an increase in Mfap5,Igf1and Fbn2 expression.

Fig. 4. Impaired induction of TH-response genes in trβ crispants

Effects of Chd1-knockdown on Hmgpi and Klf5 expression

Prophase nuclear movements in wild-type, rec8 and rec7 mutant cells.

Cdc2 expression in cultured Schwann cells.

ITSN1 recruitment stimulates cellular FN deposition to stabilize lamellipodia. ITSN1 recruitment stimulates cellular FN deposition to stabilize lamellipodia.

IGF1R is present in endometrial luminal epithelium at the receptive phase. IGF1R is present in endometrial luminal epithelium at the receptive phase. Endometrial.

Chd1 expression and CHD1 localization during mouse preimplantation development. Chd1 expression and CHD1 localization during mouse preimplantation development.

Fig. 2. Acetylation stiffens primary cilia.

Fig. 1. Generation of induced pluripotent stem cells (iPSCs) from urine cells (UC). Generation of induced pluripotent stem cells (iPSCs) from urine cells.

Fig. 1. Cell adhesion molecule expression and the aggregation of wildtype and mutant ES cells.(A) Wildtype (WT), E-cadherin null (9J), and N-cadherin null.

Proliferation on soft matrices is decoupled from myosin light chain phosphorylation, cell tractions, and focal adhesions. Proliferation on soft matrices.

Integrin β3 is dispensable for RhoA activity and contractility.

The influence of SHIP2 on cell migration in breast cancer cells.

Effect of FGF8 on R-C marker expression.

EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed cells. EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed.

Cloning and expression of rat nesprin-1.

Effects of paclitaxel and vinblastine.

BCL-3 regulates expression of stemness-associated Wnt targets.

Lack of Ctip2 is associated with impaired proliferation and delayed onset of differentiation during early stages of epidermal development. Lack of Ctip2.

Aplf downregulation does not induce cellular arrest.

Telomere cohesion is established in S phase in cohesin-ring-depleted cells. Telomere cohesion is established in S phase in cohesin-ring-depleted cells.

Aplf knockdown does not compromise DNA repair competency in iPSCs.

Generation of an inducible form of FOXN1.

Expedited loss of MacroH2A

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo. EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo.

Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

Fig. 6 The C9ORF72/SMCR8 complex regulates ULK1.

Presentation transcript:

APLF regulates genes implicated in MET during the generation of iPSCs from fibroblasts. APLF regulates genes implicated in MET during the generation of iPSCs from fibroblasts. (A) Schematic representation for the time frame (not to scale) for the generation of iPSCs. Dox, doxycycline. (B) Western blot analysis for the expression of APLF in control and Aplf-kd MEFs at different stages of reprogramming. (C) qRT-PCR analysis was performed from samples collected at different days of reprogramming, and fold changes in gene expression were calculated in Aplf-kd cells relative to those in control cells. (D) CDH1-positive cells were determined by FACS analysis at different stages of reprogramming. (E) Immunofluorescence study of the expression of CDH1 in control and Aplf-kd MEFs at day 9. Nucleus, Hoechst 33258. (F) The same set of samples as described in E were analyzed for the expression of EMT-specific genes by performing qRT-PCR. Bar graphs represent the fold change in expression relative to in cells without shRNA. Error bars are s.e.m., n=3, *P<0.05, **P<0.01, ***P<0.001 (Student's t-test). Scale bar: 20 µm. Khaja Mohieddin Syed et al. J Cell Sci 2016;129:4576-4591 © 2016. Published by The Company of Biologists Ltd