Vγ1.1Vδ6.3 TCRs transfected in TCR-deficient hybridomas are activated by anti-Vγ1.1, triggering IL-2 secretion. Vγ1.1Vδ6.3 TCRs transfected in TCR-deficient.

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Vγ1.1Vδ6.3 TCRs transfected in TCR-deficient hybridomas are activated by anti-Vγ1.1, triggering IL-2 secretion. Vγ1.1Vδ6.3 TCRs transfected in TCR-deficient hybridomas are activated by anti-Vγ1.1, triggering IL-2 secretion. TCR-deficient hybridomas transfected with the different Vγ1.1Vδ6.3 TCR variants, 2-10, 3-10, 6-10, 10-10, and 13-10, or untransfected (no TCR) or transfected with an αβTCR, were cultured in the absence (No Ab.) or presence of 5 μg/ml of anti-CD3 or 10 μg/ml of anti-Vγ1.1 Abs (Ab.). IL-2 secretion levels were measured by ELISA. Bars and error bars represent the mean and the SD of three independent experiments, respectively. A paired t test was used to analyze the statistical differences between samples treated with Ab (anti-CD3 Ab. and anti-Vγ1.1 Ab.) compared with the control sample not treated with Ab (No Ab.). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Maria Pia Negroni et al. ImmunoHorizons 2019;3:341-351 Copyright © 2019 The Authors