Ping Wu, Hong-Bo Gao, Liang-Li Zhang, Hong-Wei Xue, Wen-Hui Lin

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Phosphatidic Acid Regulates BZR1 Activity and Brassinosteroid Signal of Arabidopsis Ping Wu, Hong-Bo Gao, Liang-Li Zhang, Hong-Wei Xue, Wen-Hui Lin Molecular Plant Volume 7, Issue 2, Pages 445-447 (February 2014) DOI: 10.1093/mp/sst138 Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

Figure 1 Phosphatidic Acid Regulates AtBZR1 Activity and Brassinosteroid Signal in a PP2A-mediated Way. (A) Western-Blot analysis detected the ratio of dephosphorylation/phosphorylation BZR1 (BZR1/pBZR1) after PA treatment (left, number in red color calculated by measuring with image pro plu), BR treatment (positive control), 1-butanol treatment (middle), and PPL treatment (right, negative control labeled as DMSO). Rubisco bands in bottom panel show the equal loading. (B) BR response of Col and bzr1-1D (labeled as 1-1D) after PA treatment (left), 1-butanol treatment (middle, labeled 1-b), and Propanolol treatment (labeled PPL). Bar = 1 cm. Hypocotyl lengths were measured and relative length were shown as average±SD (n = 60). Statistical analysis were performed by a one-way AVONA module and Turkey multiple comparison methods in Statsitical Product and Service Solutions (SPSS). (C) BR and BRZ responses of Col and rcn1. Bar = 1 cm. Hypocotyl lengths were measured and relative length is shown as average±SD (right, n = 40). Statistical analysis were performed by using t-test analysis (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (D) qRT-PCR analysis of the expression of BZR1 target genes in pldζ2 mutant and PLDζ2 OE transgenic plant (left). qRT-PCR analysis of PLD genes after BR treatment (right). Means were calculated from three biological samples, and each biological sample was examined in triplicate. Error bars indicate SD. (E) Hypothetical model of PA effects in regulation of BR signal. Molecular Plant 2014 7, 445-447DOI: (10.1093/mp/sst138) Copyright © 2014 The Authors. All rights reserved. Terms and Conditions