Fig. 3 Circuits with an INHIBIT logic gate, multiple inputs (fan-in), or multiple outputs (fan-out). Circuits with an INHIBIT logic gate, multiple inputs.

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Presentation transcript:

Fig. 3 Circuits with an INHIBIT logic gate, multiple inputs (fan-in), or multiple outputs (fan-out). Circuits with an INHIBIT logic gate, multiple inputs (fan-in), or multiple outputs (fan-out). (A) Two-input Disassembly INHIBIT gate implemented in a pair of G-NR (R1) and G-NF (F1). X1 removes a preformed DNA bond via strand displacement, whereas X2 forms a different DNA bond. Disassembly of the R1–F1 dimer is controlled by INHIBIT logic (X1 AND NOT X2) based on the two competing reactions. Actions of single-particle INHIBIT gate are shown. Scale bar, 1 μm. (B) Six-input Disassembly gate implemented in a pair of G-NF (R2) and G-NF (F2). The receptor (R2) and the floater (F2) are connected by three distinct DNA bonds, and the cleavage of each bond is controlled by two-input OR logic. (C) Two-input Disassembly AND gate with three outputs. This circuit evaluates two-input AND logic and generates three different mobile floaters—R-NF (F3), G-NF (F4), and B-NF (F5)—as outputs. Three representative receptors that release F3, F4, and F5 are labeled as R3(i), R3(ii), and R3(iii), respectively. Dashed arrows in time traces indicate disassembly reactions. The range of y-axis values in the time traces is 0 to 250. ∧, ∨, and ¬ denote the logic symbols for AND, OR, and NOT, respectively. Legends are represented as truth tables. DNA sequences and experimental conditions are listed in tables S2 and S3. Experiments were carried out at 25°C in 1× PBS buffer. Jinyoung Seo et al. Sci Adv 2019;5:eaau2124 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).