A B D C E F Supplementary Figure S4. Epping et al. IP: flag input IgG

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A B D C E F Supplementary Figure S4. Epping et al. IP: flag input IgG 150 100 75 50 flag- TSPYL2 SIN3A 37 25 20 FL FL 1 2 3 FL FL 1 2 3 TSPYL2 Interaction of TSPYL2 domains with SIN3A A B Gal4-luciferase 0.1 0.2 0.3 0.4 0.5 0.6 Gal4 Gal4- T2 (FL) T2 (1) T2 (2) T2 (3) Relative luciferase activity IP REST (1-323) REST (322-1097) flag-REST (1-323) TSPYL2 CoREST SIN3A (322-1097) IgG flag input D C 1 322 841 1097 1-1097 1-323 322-1097 322-841 841-1097 E IP REST (322-841) REST (841-1097) IgG flag input flag-REST (322-841) (841-1097) TSPYL2 SMAD4 TSPYL2 actin GFP TSPYL2#1 TSPYL2#2 SMAD4#1 shRNA F