Diaphanous regulates the presynaptic actin cytoskeleton.

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Diaphanous regulates the presynaptic actin cytoskeleton. Diaphanous regulates the presynaptic actin cytoskeleton. Representative projections from larval NMJs expressing an actin-GFP transgene in a wild type ( A , B ), a dia 2/2 mutant animal ( C , D ), and a dlar 5.5 mutant animal ( E , F ). Images show either a live image of actin-GFP or the same NMJ after fixation and staining with anti-HRP to reveal the presynaptic membrane, as indicated. G , Graph represents the number of synaptic actin foci divided by the number of boutons. Error bars represent SEM. Scale bar, 10 μm. Asterisks indicate statistically significant differences compared with wild type. Catherine Pawson et al. J. Neurosci. 2008;28:11111-11123 ©2008 by Society for Neuroscience