Fig. 1 Nanowire-induced electrostatic collection of urine EVs followed by in situ extraction of EV-encapsulated miRNAs. Nanowire-induced electrostatic.

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Fig. 1 Nanowire-induced electrostatic collection of urine EVs followed by in situ extraction of EV-encapsulated miRNAs. Nanowire-induced electrostatic collection of urine EVs followed by in situ extraction of EV-encapsulated miRNAs. (A) Schematic illustrations for urine EV collection and in situ extraction of urine EV–encapsulated miRNAs using a nanowire-anchored microfluidic device. (B) A schematic illustration (gray rods, nanowires; transparent cyan areas, PDMS) and an inset illustration on the lower left showing a cross-sectional image (yellow and blue represent nanowires and PDMS, respectively) for buried nanowires after poring, curing, and peeling off PDMS, and a vertical cross-sectional FESEM image of buried nanowires; nanowires and PDMS are highlighted as yellow and blue, respectively, and the white dotted line indicates a PDMS edge. Scale bar, 1 μm. (C) A schematic illustration and an inset illustration on the lower left showing a cross-sectional image for growing nanowires from the buried nanowires (nanowire-embedded PDMS), and a vertical cross-sectional image of the nanowire-embedded PDMS. Scale bar, 1 μm. (D) A schematic illustration and an inset illustration on the lower left showing a cross-sectional image for bonding the nanowire-embedded PDMS substrate to the microfluidic herringbone-structured PDMS substrate, an image of the nanowire-anchored microfluidic device (bonding the nanowire-embedded PDMS and the microfluidic herringbone-structured PDMS substrates) with PEEK tubes for an inlet and an outlet (scale bar, 1 cm), and a laser micrograph of the microfluidic herringbone structure on PDMS (scale bar, 1 mm). (E) A schematic illustration of the nanowire-embedded PDMS (gray rods, nanowires; transparent cyan areas, PDMS), and an overview of FESEM image for the nanowire-embedded PDMS (scale bar, 1 μm) after being exposed to lysis buffer. (F) A schematic illustration of nanowires on the Si substrate (gray rods, nanowires; dark cyan areas, Si substrate; faded cyan areas, Cr layer), and an overview of FESEM image for the nanowires on the Si substrate after being exposed to lysis buffer. Scale bar, 1 μm. Takao Yasui et al. Sci Adv 2017;3:e1701133 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).