Engraftment of ROSAKIT D816V-Gluc cells in NSG mice.

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Engraftment of ROSAKIT D816V-Gluc cells in NSG mice. Engraftment of ROSAKIT D816V-Gluc cells in NSG mice. Increasing numbers of ROSAKIT D816V-Gluc cells (1×106, 5×106 or 10×106) were injected into the tail vein of NOD-SCID IL-2Rγ-/- (NSG) mice (3 groups; 6 mice per group) 24 h after irradiation at 2.5 Gy from a cesium-137 source.113 After 10 weeks, engraftment was assessed using (A) quantitative measurements of Gluc activity in plasma and (B) in vivo bioluminescence imaging (IVIS) on engrafted mice. Ten weeks after engraftment, mice were sacrificed and (C) bone marrow and (D) spleen sections from the three groups of mice were stained by immunohistochemistry with an anti-human tryptase antibody. Staining was visualized by a Histomouse Kit, showing human MC with a brownish stain, which massively invaded the bone marrow and to a lesser extent the spleen. (C) and (D) are from one representative mouse from the group injected with 10×106 ROSAKIT D816V-Gluc cells. Original magnification, ×100. Michel Arock et al. Haematologica 2018;103:1760-1771 ©2018 by Ferrata Storti Foundation