Using CLARITY to clear and image other organs.

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Using CLARITY to clear and image other organs. Using CLARITY to clear and image other organs. A, Mouse spleen before and after CLARITY and low- and high-magnification images of CD34 immunohistochemistry on cleared tissue. B, Cleared mouse intestine stained with propidium iodide. C, Cleared mouse kidney labeled for smooth muscle actin. D, Mouse testis stained with propidium iodide. E, Mouse lung immunolabeled for collagen. F, Section of mouse muscle stained with propidium iodide. All fluorescent images were collected using a LaVision Biotec light sheet microscope except for the high-magnification image of the spleen, which was captured using a Zeiss confocal microscope. Jonathan R. Epp et al. eneuro 2015;2:ENEURO.0022-15.2015 ©2015 by Society for Neuroscience