Comparison of gut microbiota alpha diversity in different preservatives based on 16S rRNA gene V3-V4 amplicon sequencing. Comparison of gut microbiota alpha diversity in different preservatives based on 16S rRNA gene V3-V4 amplicon sequencing. Alpha diversity analysis on amplicon sequence variant (ASV) (A) and genus (B) levels. Principal-coordinate plot using unweighted (C) and weighted (D) UniFrac distances, with sample clustering by individual subjects. The PC1 and PC2 axes represent the first two principal coordinates. KW test, Kruskal-Wallis test. Zigui Chen et al. mSystems 2019; doi:10.1128/mSystems.00271-18