Transforming growth factor-β1 increases albumin permeability of isolated rat glomeruli via hydroxyl radicals Ram Sharma, Ashwani Khanna, Mukut Sharma,

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Transforming growth factor-β1 increases albumin permeability of isolated rat glomeruli via hydroxyl radicals Ram Sharma, Ashwani Khanna, Mukut Sharma, Virginia J. Savin Kidney International Volume 58, Issue 1, Pages 131-136 (July 2000) DOI: 10.1046/j.1523-1755.2000.00148.x Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 1 Effect of various amounts of transforming growth factor-β1 (TGF-β1) on glomerular albumin permeability (Palb). Rat glomeruli were incubated with or without 1 to 10 ng/mL of TGF-β1 for 15 minutes at 37°C, and Palb was measured. As shown, 1 and 2.5 ng/mL of TGF-β1 had very little effect on Palb (N = 17 and 20, respectively) compared with control glomeruli (N = 25). As shown, 5 and 10 ng/mL of TGF-β1 caused a significant increase (*P < 0.01) in Palb (N = 20 and 23, respectively). Kidney International 2000 58, 131-136DOI: (10.1046/j.1523-1755.2000.00148.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 2 Time course of TGF-β1–mediated increase in glomerular Palb. Rat glomeruli were incubated with 10 ng/mL of TGF-β1 for up to 60 minutes, and the increase in Palb was measured. TGF-β1 10 ng/mL caused a significant increase in Palb (*P < 0.01) within 15 minutes (N = 23), and this effect of TGF-β1 increased further at 30 (N = 24), 45 (N = 25), and 60 minutes (N = 21) of incubation. Kidney International 2000 58, 131-136DOI: (10.1046/j.1523-1755.2000.00148.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 3 Effect of reactive oxygen species (ROS) scavengers on TGF-β1 mediated increase in glomerular Palb. Rat glomeruli were incubated in isolation medium, in control medium (4N HCl), with 10 ng/mL of TGF-β1 and TGF-β1 + SOD, or DMTU and SOD or DMTU alone for up to 60 minutes. (A) TGF-β1 caused a significant increase (*P < 0.01) in Palb (N = 15), and SOD (200U/mL) blocked this effect (N = 15; shown by two asterisks. Four N HCl and SOD alone had no effect on Palb (N = 15 and N = 22, respectively). (B) TGF-β1 caused a significant increase (*P < 0.01) in Palb (N = 15), and 1 mmol/L of DMTU (N = 15) also blocked the TGF-β1–mediated increase in Palb, as shown by two asterisks, and DMTU alone had no effect on Palb (N = 15). Kidney International 2000 58, 131-136DOI: (10.1046/j.1523-1755.2000.00148.x) Copyright © 2000 International Society of Nephrology Terms and Conditions