Levels of apoptosis in human granulosa cells seem to be comparable after therapy with a gonadotropin-releasing hormone agonist or antagonist Franca Giampietro,

Slides:

Advertisements

Similar presentations

The effectiveness of gonadotropin-releasing hormone antagonist in poor ovarian responders undergoing in vitro fertilization: a systematic review and meta-analysis

Advertisements

Isolation of spermatozoa with low levels of fragmented DNA with the use of flow cytometry and sorting Sofia C. Ribeiro, Ph.D., Gideon Sartorius, M.D.,

Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress Reda.

Expression of GRIM-19 in missed abortion and possible pathogenesis

Ultrastructure of human oocytes of different maturity stages and the alteration during in vitro maturation Yong-jie Yang, M.Sc., Yan-jun Zhang, Ph.D.,

Evaluation of poly(ADP-ribose) polymerase cleavage (cPARP) in ejaculated human sperm fractions after induction of apoptosis Reda Z. Mahfouz, M.D., Rakesh.

Gonadotropin-releasing hormone agonist trigger in oocyte donors co-treated with a gonadotropin-releasing hormone antagonist: a dose-finding study Thi.

The relative effects of hormones and relationship factors on sexual function of women through the natural menopausal transition Lorraine Dennerstein,

Can pregnancy rate be improved in gonadotropin-releasing hormone (GnRH) antagonist cycles by administering GnRH agonist before oocyte retrieval? A prospective,

Triggering with human chorionic gonadotropin or a gonadotropin-releasing hormone agonist in gonadotropin-releasing hormone antagonist-treated oocyte donor.

Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates Chun-Chia Huang, M.Sc., David.

1,500 IU human chorionic gonadotropin administered at oocyte retrieval rescues the luteal phase when gonadotropin-releasing hormone agonist is used for.

Characterization of human sperm populations using conventional parameters, surface ubiquitination, and apoptotic markers Sandra Varum, B.Sc., Carla Bento,

Proportional change of CD4+CD25+ regulatory T cells after lymphocyte therapy in unexplained recurrent spontaneous abortion patients Hui Yang, M.D., Ph.D,

Dienogest enhances autophagy induction in endometriotic cells by impairing activation of AKT, ERK1/2, and mTOR JongYeob Choi, Ph.D., MinWha Jo, M.S.,

Mitochondrial function is altered in articular chondrocytes of an endemic osteoarthritis, Kashin–Beck disease J.T. Liu, X. Guo, W.J. Ma, Y.G. Zhang,

Isolation of spermatozoa with low levels of fragmented DNA with the use of flow cytometry and sorting Sofia C. Ribeiro, Ph.D., Gideon Sartorius, M.D.,

Dorien Van Saen, Ph. D. , Ellen Goossens, Ph. D. , Joeri L. Aerts, Ph

Apoptosis and ultrastructural assessment after cryopreservation of whole human ovaries with their vascular pedicle Belen Martinez-Madrid, V.M.D., Ph.D.,

Hee-Jun Chi, Ph. D. , Jung-Jin Koo, M. D. , Soon-Young Choi, M. Sc

The apoptotic pathway in fertile and subfertile men: a case-control and prospective study to examine the impact of merocyanine 540 bodies on ejaculated.

Effects of retinoic acid on the inner cell mass in mouse blastocysts

Evidence of modified nuclear protein matrix in human spermatozoa with fragmented deoxyribonucleic acid Rebeca Santiso, Ph.D., Lourdes Muriel, Ph.D.,

Role for the endometrial epithelial protein MFG-E8 and its receptor integrin αvβ3 in human implantation: results of an in vitro trophoblast attachment.

CD44-mediated neutrophil apoptosis in the rat

Diagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility Cinthia M.

Morphometric and ultrastructural analysis of human granulosa cells after gonadotrophin- releasing hormone agonist or antagonist L. Centurione, F. Giampietro,

A new step toward the artificial ovary: survival and proliferation of isolated murine follicles after autologous transplantation in a fibrin scaffold

Dong-Wook Park, M. Sc. , Taesup Cho, M. Sc. , Mi Ran Kim, M. D

Morphologic, ultrastructural, and biochemical identification of apoptosis in vitrified- warmed mouse ovarian tissue Tahere Mazoochi, M.Sc., Mojdeh Salehnia,

Evaluation of a human ovarian follicle isolation technique to obtain disease-free follicle suspensions before safely grafting to cancer patients Michelle.

Measurement of sperm DNA fragmentation using bright-field microscopy: comparison between sperm chromatin dispersion test and terminal uridine nick-end.

Robert F. Casper, M.D. Fertility and Sterility

Weihong Hu, M. D. , Ph. D. , Dennis Marchesi, Ph. D. , Jie Qiao, M. D

Needle immersed vitrification can lower the concentration of cryoprotectant in human ovarian tissue cryopreservation Zhun Xiao, M.D., Yan Wang, Ph.D.,

Preservation of human ovarian follicles within tissue frozen by vitrification in a xeno-free closed system using only ethylene glycol as a permeating.

Anna Kékesi, Edit Erdei, M. D. , Ph. D. , Miklós Török, M. D. , Ph. D

Ultrashort flare gonadotropin-releasing hormone (GnRH) agonist/GnRH antagonist protocol: a valuable tool in the armamentarium of ovulation induction for.

Patricia Miyuki Tsuribe, Ph. D. , Carlos Alberto Monte Gobbo, M. D

The effectiveness of gonadotropin-releasing hormone antagonist in poor ovarian responders undergoing in vitro fertilization: a systematic review and meta-analysis

Does stimulation with human gonadotropins and gonadotropin-releasing hormone agonist enhance and accelerate the developmental capacity of oocytes in human.

Low-molecular-weight heparins induce decidual heparin-binding epidermal growth factor–like growth factor expression and promote survival of decidual cells.

Differential pH in embryo culture

Treatment of Ovarian Hyperstimulation Syndrome Utilizing a Dopamine Agonist and Gonadotropin Releasing Hormone antagonist N. Rollene, M. Amols, S. Hudson,

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Association of sperm apoptosis and DNA ploidy with sperm chromatin quality in human spermatozoa Reda Z. Mahfouz, M.D., Rakesh K. Sharma, Ph.D., Tamer.

Gwo-Jang Wu, M. D. , Ph. D. , Fung-Wei Chang, M. D. , Shang-Sen Lee, M

Fragmentation of DNA in morphologically normal human spermatozoa

Lysed cell removal promotes frozen–thawed embryo development

Exogenous androstenedione induces formation of follicular cysts and premature luteinization of granulosa cells in the ovary Yuki Okutsu, M.D., Masanori.

Higher PDCD4 expression is associated with obesity, insulin resistance, lipid metabolism disorders, and granulosa cell apoptosis in polycystic ovary syndrome

Natalia Rougier, M. D. , Heydy Uriondo, M. Sc. , Sergio Papier, M. D

Angiotensin II induces apoptosis in human mural granulosa-lutein cells, but not in cumulus cells Elisa Acosta, M.Sc., Óscar Peña, M.Sc., Frederick Naftolin,

Effect of gonadotropin-releasing-hormone-induced hypogonadism on insulin action as assessed by euglycemic clamp studies in men Subodhsingh Chauhan, M.D.,

Quantitative evaluation of spermatozoa ultrastructure after acupuncture treatment for idiopathic male infertility Jian Pei, Ph.D., Erwin Strehler, M.D.,

Redefining the relationship between sperm deoxyribonucleic acid fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted.

Stimulation of mitochondrial embryo metabolism by dichloroacetic acid in an aged mouse model improves embryo development and viability Nicole O. McPherson,

Seul Ki Kim, M. D. , Byung Chul Jee, M. D. , Ph. D. , Seok Hyun Kim, M

Follicular phase serum levels of luteinizing hormone do not influence delivery rates in in vitro fertilization cycles down-regulated with a gonadotropin-releasing.

Cost-effectiveness comparison between pituitary down-regulation with a gonadotropin- releasing hormone agonist short regimen on alternate days and an antagonist.

Current evaluation of amenorrhea

Gonadotropin-releasing hormone agonists for prevention of chemotherapy-induced ovarian damage: prospective randomized study Ahmed Badawy, M.D., Aboubakr.

Nerve fibers and menstrual cycle in peritoneal endometriosis

Soluble HLA-G in the peritoneal fluid of women with endometriosis

In vitro viability and secretory capacity of human luteinized granulosa cells after gonadotropin-releasing hormone agonist trigger of oocyte maturation

Nicotine induces apoptosis in TM3 mouse Leydig cells

L-Carnitine decreases DNA damage and improves the in vitro blastocyst development rate in mouse embryos Hussein Abdelrazik, M.D., Rakesh Sharma, Ph.D.,

Claire E. Beyer, B. Sc. , Bettina Kayler, B. M. Sc

Yinghui Ye, M. D. , Ph. D. , Chenming Xu, PhD. , Yuli Qian, B. Sc

Alinne Colin, M. D. , Gerardo Barroso, M. D. , M. Sc

Presentation transcript:

Levels of apoptosis in human granulosa cells seem to be comparable after therapy with a gonadotropin-releasing hormone agonist or antagonist Franca Giampietro, M.D., Ph.D., Silvia Sancilio, Ph.D., Gian Mario Tiboni, M.D., Rosa Alba Rana, M.D., Roberta Di Pietro, M.D. Fertility and Sterility Volume 85, Issue 2, Pages 412-419 (February 2006) DOI: 10.1016/j.fertnstert.2005.08.020 Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Results of flow cytometric analysis of granulose cells of one representative experiment (GnRH-agonist-treated women). Early apoptotic cell populations (Annexin V+/PI−) can be discriminated from vital (Annexin V−/PI−) or necrotic cells (Annexin+/PI+) according to their fluorescence emission. Cells in quadrant D4 are apoptotic. Quadrants D1 and D2 represent dead and damaged cells, respectively, and vital cells are situated in quadrant D3. Giampietro. GnRH agonist/antagonist and apoptosis. Fertil Steril 2006. Fertility and Sterility 2006 85, 412-419DOI: (10.1016/j.fertnstert.2005.08.020) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Phase contrast and fluorescent micrographs showing granulosa cell apoptotic (A) and normal (N) nuclei assayed with the terminal deoxynucleotidyl transferase (TdT)-mediated nick-end labeling (TUNEL) technique. Original magnification, ×40. Giampietro. GnRH agonist/antagonist and apoptosis. Fertil Steril 2006. Fertility and Sterility 2006 85, 412-419DOI: (10.1016/j.fertnstert.2005.08.020) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 Deoxyribonucleic acid histogram of granulose cells (GCs) stained with PI and analyzed with flow cytometry. The inset shows a representative field of GC nuclei counterstained with 6-diamino-2-phenylindole (DAPI) and observed with fluorescent microscopy. No evidence of typical micronuclei was observed. Giampietro. GnRH agonist/antagonist and apoptosis. Fertil Steril 2006. Fertility and Sterility 2006 85, 412-419DOI: (10.1016/j.fertnstert.2005.08.020) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 4 (A) Electron micrograph showing a typical granulose cell (GC) with a large, round, and eccentric nucleus (Nu) with the presence of dispersed chromatin and distinctly visible nucleoli (white arrows). Note abundant osmiophilic lipid droplets scattered throughout the cytoplasm (black arrows). Well-preserved mitochondria (M) with complex tubular cristae are often located adjacent to electron-dense droplets. Original magnification, ×7,000; ×50,000 in inset. (B) Apoptotic GC with the aspect of peripheral chromatin condensation and margination. Original magnification, ×4,000. (C) A normal GC which is removing by phagocytosis a highly condensed nucleus (black arrow) of an apoptotic cell. Original magnification, ×4,000. Giampietro. GnRH agonist/antagonist and apoptosis. Fertil Steril 2006. Fertility and Sterility 2006 85, 412-419DOI: (10.1016/j.fertnstert.2005.08.020) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions