Stress resistance comparisons.

Slides:



Advertisements
Similar presentations
BY4742  yhr087w YPDYP25YPD KCl 1MYP30 S.1 Defects of the YHR087W deletion mutant under osmotic stress conditions. Growth of the BY4742 and  yhr087w strains.
Advertisements

Virulence of aggregate-forming and nonaggregate strains of Candida auris compared to C. albicans in Galleria mellonella larvae at 30°C (upper panel) and.
Butanol is likely to inhibit translation via effects on eIF2B.
Sgs1Δ103−322 physically interacts with Top3–Rmi1.
Cg-OxyR controls catalase transcription, recovery from lag phase, survival to H2O2 stress, and H2O2 scavenging rate. Cg-OxyR controls catalase transcription,
Volume 36, Issue 5, Pages (December 2009)
Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ.
Putative ammonia transporters are not required for ammonia release during N-acetylglucosamine pH neutralization. Putative ammonia transporters are not.
Robustness of the cellular system in sake yeast strains.
Dibutyryl cAMP does not rescue staurosporine-induced filamentation of a cyr1Δ/cyr1Δ mutant. Dibutyryl cAMP does not rescue staurosporine-induced filamentation.
Staurosporine-induced filamentation requires Cyr1 and PKA
Gap2 is required for growth on phenylalanine as the sole source of nitrogen. Gap2 is required for growth on phenylalanine as the sole source of nitrogen.
The pho4Δ mutant is less virulent than the WT strain in intranasal (A to C) and intravenous (D to F) models of cryptococcosis. The pho4Δ mutant is less.
Ras1 is a specific target of Ram1.
The virulence of Candida species in Galleria mellonella larvae at 37°C is species specific. The virulence of Candida species in Galleria mellonella larvae.
Cell wall hydrolytic activities of Andhra lysins.
Sir2 Blocks Extreme Life-Span Extension
FLC treatment results in an increase in ploidy in a significant fraction of cells. FLC treatment results in an increase in ploidy in a significant fraction.
Quiescence of the E. coli CFT073 original clinical isolate on glucose plates. Quiescence of the E. coli CFT073 original clinical isolate on glucose plates.
Changing to a coconut oil-containing diet reduces preexisting GI colonization by C. albicans. Changing to a coconut oil-containing diet reduces preexisting.
Sensitivity of E. coli alkA tag PolIV-proficient and -deficient mutants to different alkylating agents. Sensitivity of E. coli alkA tag PolIV-proficient.
Side-view and apical-view projections of mutant and validation strain biofilms. Side-view and apical-view projections of mutant and validation strain biofilms.
Volume 14, Issue 2, Pages (August 2008)
Prenylation and postprocessing enzymes Ram1, Rce1, Ste24, and Ste14 play a role in mating filament formation during C. neoformans sexual development. Prenylation.
Fur homodimerization and functional assays
Analyzing Fission Yeast Multidrug Resistance Mechanisms to Develop a Genetically Tractable Model System for Chemical Biology  Shigehiro A. Kawashima,
Candida albicans levels were decreased in the mouth and stool of healthy human volunteer(s) when teeth were brushed more often. Candida albicans levels.
Antibiofilm effects of zinc pyrithione and silver sulfadiazine individually and in combination against S. aureus, A. baumannii, and P. aeruginosa. Antibiofilm.
S. pneumoniae GapA is sensitive to oxygen and H2O2 exposure.
Stress-protective roles of C. auris Hog1.
RNA-Seq analysis of CYR1 cells in the adaptation to acid pH.
Competitive growth analysis of WT and ΔnsiR4.
Cell density and secreted enzymes contribute to aggregate invasive growth. Cell density and secreted enzymes contribute to aggregate invasive growth. (A)
Growth of Salmonella and E. coli in egg white.
Screening for strains haploinsufficient for growth in the presence of SMM. Each panel shows a diagram of the dilution series performed as well as plates.
Mice Lacking TLR11 Exhibit Variable Salmonella typhi Susceptibility
E. coli CFT073 and E. coli MG1655 persistence.
Prevention of quiescence by human urine and amino acids.
Sir2 Blocks Extreme Life-Span Extension
Proteus mirabilis strain HI4320 (WT) and isogenic mutants for dsdA, dsdC, or dsdX were cultured in LB broth overnight and diluted 1:100 into fresh LB (A),
Volume 49, Issue 5, Pages (March 2013)
Expression of all the GAP genes is under control of the Csy1 sensor and regulated by nitrogen sources. Expression of all the GAP genes is under control.
ToxT production profiles of single-SNP strains and double-, triple-, and quadruple-SNP combination strains. ToxT production profiles of single-SNP strains.
Susceptibility to hydrogen peroxide.
Uptake of amino acids by C. albicans.
The effect of Bos1 mutations on yeast growth.
Construction and analysis of C. auris hog1Δ cells.
Sequence comparisons of fungal HOG1 orthologues.
Intranasal immunization of mice with S
TcpA production profiles of single-SNP strains and double-, triple-, and quadruple-SNP combination strains. TcpA production profiles of single-SNP strains.
CRISPRi-based repression of the essential gene HSP90 in C. albicans.
CT production profiles of single-SNP strains and double-, triple-, and quadruple-SNP combination strains. CT production profiles of single-SNP strains.
Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2. Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2. (A)
dCas9-mediated repression of transcription.
EGCG inhibits cell survival and growth of EFT cell lines but not of HBMECs. A, dose-responsive curves of fractional survival of EFT cells (TC32 and TC71)
CDDO-Me induces apoptosis independent of Bcl-2 level as well as p53 status in human NSCLC cells. CDDO-Me induces apoptosis independent of Bcl-2 level as.
Titration of acute-phase tick-borne disease (TBD) samples in indirect immunofluorescence assay (IFA) to determine optimal screening dilutions. Titration.
SlRd2 expression confers LiCl resistance in yeast and its dimerization is required for its biological function. SlRd2 expression confers LiCl resistance.
SskA and sakA are essential for adaptation to cell wall, osmotic, and salt stress. sskA and sakA are essential for adaptation to cell wall, osmotic, and.
Mig1 influences the TOR and cell wall remodeling pathways.
CRISPRi repression with dCas9-repressor fusion constructs.
Distribution of selected traits across the >4,000 strains in the most recent version of the database, including cell shape (A), spore formation (B), motility.
Dose-dependent growth inhibition assays of fluconazole and DM262.
Production of ade2Δ/ade2Δ mutants by the Candida CRISPR system.
Growth with N-acetylglucosamine rapidly raises the environmental pH and releases detectable ammonia. Growth with N-acetylglucosamine rapidly raises the.
Phylogenetic analysis of complete Fusobacterium genomes.
Microscopic imaging of different C
Mixed head-to-head competitions.
Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen. Proteus mirabilis can utilize d-serine as a sole source of carbon and nitrogen.
Identification of bacterial species producing the anti-Candida molecules. Identification of bacterial species producing the anti-Candida molecules. (A)
Presentation transcript:

Stress resistance comparisons. Stress resistance comparisons. (A) C. auris has a unique stress resistance profile. Exponentially growing Candida strains were spotted in serial dilutions onto YPD agar plates containing the indicated additives and incubated for 24 or 48 h at 30°C. (B) C. auris is more resistant than C. albicans to H2O2. Exponentially growing Candida strains were treated with 25 mM H2O2, and cell survival at the indicated times was calculated as described in Materials and Methods. (C) C. auris is sensitive to combinatorial H2O2 and cationic stress. Exponentially growing strains and 10-fold dilutions thereof were spotted onto the indicated plates and incubated at 30°C for 24 h. Alison M. Day et al. mSphere 2018; doi:10.1128/mSphere.00506-18