Intellicount recapitulates analysis of synaptogenesis over time in cultured hippocampal neurons. Intellicount recapitulates analysis of synaptogenesis.

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Intellicount recapitulates analysis of synaptogenesis over time in cultured hippocampal neurons. Intellicount recapitulates analysis of synaptogenesis over time in cultured hippocampal neurons. A, Upper panels: representative images taken at increasing numbers of days in culture. Lower panels, corresponding traces provided by Intellicount (red, correlated puncta synapsin traces; green, segmented dendrite traces in MAP2 channel; blue, trace of soma segmented from MAP2 channel). Display of the MAP2 channel was deselected in the lower panels facilitate visualization of puncta. A total of 29–33 images were obtained from randomly sampled fields distributed evenly over two coverslips each obtained from two independent cultures. B–D, Quantification and graphical representation of (B) synapse number, (C) mean fluorescence intensity, and (D) area provided by Intellicount with minor adjustments in Adobe Illustrator (renaming of “rounds” to appropriate conditions and thickening of stroke width for representation of graph and bar lines). Colors displayed are default in the program. Data are displayed as mean ± SEM. Numbers within bars indicate the number of images processed from that time point. J. A. Fantuzzo et al. eNeuro 2017;4:ENEURO.0219-17.2017 ©2017 by Society for Neuroscience