The sesquiterpene lactone PN up-regulates MDM2 and p53 proteins.

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The sesquiterpene lactone PN up-regulates MDM2 and p53 proteins. The sesquiterpene lactone PN up-regulates MDM2 and p53 proteins. A, chemical structure of PN. B, Western blots of cytoplasmic and nuclear extract proteins from ZR-75-1 cells treated with PN at increasing concentrations for 3 h and probed with the indicated antibodies (right). Paxillin and poly(ADP-ribose) polymerase (PARP) immunostaining served as protein loading controls and as controls for cytoplasmic and nuclear extract integrity. C, Western blotting done as described in A but with 15 μmol/L PN and increasing treatment durations. Y.N. Vashisht Gopal et al. Mol Cancer Ther 2009;8:552-562 ©2009 by American Association for Cancer Research